Sequences from confirmation amplicon


Confirmation sequences for line 121H11 and hit At1g62490 are shown.
Line ID 121H11
Locus At1g62490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23131659
Primers used for PCR ER50 - 8474, show primer details
   
Sequence Name 20-K119249-0022-121-H11C-ER50
GenBank Accession LN495441 [GenBank]
Sequencing primer gene specific primer, ER50: 5'-TGATGACCGTGAAGATTAAGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K119249-0022-121-H11C-ER50
ATTATCGTTATAACTAGGGTTCTTGAAATTTTCATGAAACTCTTGTTGCATTATTGTCAA
ATCCTTTTGGCAAATAACATGCAAATATATAGTTGAAGCTTAAATGTGACCCGTGACTCT
TGTATGGGTGTTAACCACTACGCACATAAATAGGTATAGTGTTTATAAGTGTAGTAGATT
TGATTATTTGTTATGGTACGTACAAACGAACATGTGTAAGGCAAGAAAATGAAGAAAAAA
GAAGATGTTCCAAACTTCGAGAAATATTGGAGCATTTTATTGCGTTACAACTAAAGGTTT
TTTGGATCGTTGTTGGTCCGATGCAAACTTCAAGGTCAAGCCCGTGACTACAAAGTATAT
ATCAGATTATGATCACTGTCTCCGAACTAAAGAGTCCGTGAAATTGTTTGTTCTCTTATG
TATTTAACTTTATTTCTAATGACATTTTGTTGAAACTTGAAAAGACTTGACAACAAACAG
TGAACGCTTCTTAATAACGTAGAGGGTTTCGTTGTCCGTACAATTGAATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 04-K119248-0022-121-H11C-8409
GenBank Accession LN488133 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K119248-0022-121-H11C-8409
ATTTACATTGAATATTCAATTGTACGGACAACGAAACCCTCTACGTTATTAAGAAGCGTT
CACTGTTTGTTGTCAAGTCTTTTCAAGTTTCAACAAAATGTCATTAGAAATAAAGTTAAA
TACATAAGAGAACAAACAATTTCACGGACTCTTTAGTTCGGAGACAGTGATCATAATCTG
ATATATACTTTGTAGTCACGGGCTTGACCTTGAAGTTTGCATCGGACCAACAACGATCCA
AAAAACCTTTAGTTGTAACGCAATAAAATGCTCCAATATTTCTCGAAGTTTGGAACATCT
TCTTTTTTCTTCATTTTCTTGCCTTACACATGTTCGTTTGTACGTACCATAACAAATAAT
CAAATCTACTACACTTATAAACACTATACCTATTTATGTGCGTAGTGGTTAACACCCATA
CAAGAGTCACGGGTCACATTTAAGCTTCAACTATATATTTGCATGTTATTTGCCAAAAGG
ATTTGACAATAATGCAACAAGAGTTTCATTGAAAATTTCAAGAACCCTAGTTAATAACGA
TAATGGGTTCGAGAGTTTTGGCTTCCTTCTTTGTTTTCTTAAT

Last Updated on 06.12.2018 16:11