Sequences from confirmation amplicon


Confirmation sequences for line 621H03 and hit At1g63640 are shown.
Line ID 621H03
Locus At1g63640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23594097
Primers used for PCR CH82 - 8474, show primer details
   
Sequence Name 04-K107108-0022-621-H03C-8409
GenBank Accession LN487941 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K107108-0022-621-H03C-8409
GCCATTTACAATTGAATATTTCCAGTCGTCAGACAAAAACCATGATCAGAAAAGTGTTGC
ACCCAGAAGTGTTTAAAACGAGGCTAATTTTAATCTAAACATATGCCTACACAGAATTGG
TTCGACTCATTAAAGGAAAAGTGGCAATACGACAACCACTTCATCTATATGACATGGATA
ATTCTATGTAAACCAGCATGTCTTTCCATATTTAAGAAATCAACTTTTTCCACAGAAATG
AAGAATAATATCCATGAAAACATAACAGACCTGTTCTATATCTGAAACTTCGAACCTAGG
CAGGGCCATTTCATCCATAGCAGGTCAGAA
   
Sequence Name 20-K107113-0022-621-H03C-CH82
GenBank Accession LN495235 [GenBank]
Sequencing primer gene specific primer, CH82: 5'-TTTCTGACTGCTATGGATGAAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K107113-0022-621-H03C-CH82
GGTCTGTTATGTTTTCTGGATATTATTCTTCATTTCTGTGGAAAAAGTTGATTTCTTAAA
TATGGAAAGACATGCTGGTTTACATAGAATTATCCATGTCATATAGATGAAGTGGTTGTC
GTATTGCCACTTTTCCTTTAATGAGTCGAACCAATTCTGTGTAGGCATATGTTTAGATTA
AAATTAGCCTCGTTTTAAACACTTCTGGGTGCAACACTTTTCTGATCATGGTTTTTGTCT
GACGACTGGAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAAGGTTATT

Last Updated on 06.12.2018 16:11