Sequences from confirmation amplicon


Confirmation sequences for line 155B11 and hit At1g63820 are shown.
Line ID 155B11
Locus At1g63820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23683028
Primers used for PCR 04fy - 8474, show primer details
   
Sequence Name 59-K111411-0022-155-B11C-04fy
GenBank Accession LN510034 [GenBank]
Sequencing primer gene specific primer, 04fy: 5'-AGTTCACACAAGCTTCTTCTTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K111411-0022-155-B11C-04fy
TTAAAAAAAGTAATAATTATCAGTTATTTACTTAATCTCAATTTAAATACAATTTATTAT
ACTTGTATAAAAAGATGAAAAAGTAAGGTTTGTAATTTAGGAATCAACTTGAATTTTACT
TACCCAAAGGTCGTCGTCGTTTTCTTGTCTAGTGAAAGAAGAAGCAATCTTGGGGTTCTC
AAACACTTCGTCGTTTCTTGCAAATCTGCCACGTACTCTTGGTCTGTTGTCCGCCAACGT
TTTCCGGCATGCATACTTCAATTTTTGCATTTAATATATAAGAAAATTATAACGGTGCAA
ACCAAAATTACAAATGTAAAGTTGTAATTTGTATAAAATTGTAAAACAATTATTTAATTA
ATTGAATATGTGCATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAT
   
Sequence Name 53-K111410-0022-155-B11C-8409
GenBank Accession LN508190 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K111410-0022-155-B11C-8409
TCATTGAATAATGCACATATTCAATTAATTAAATAATTGTTTTACAATTTTATACAAATT
ACAACTTTACATTTGTAATTTTGGTTTGCACCGTTATAATTTTCTTATATATTAAATGCA
AAAATTGAAGTATGCATGCCGGAAAACGTTGGCGGACAACAGACCAAGAGTACGTGGCAG
ATTTGCAAGAAACGACGAAGTGTTTGAGAACCCCAAGATTGCTTCTTCTTTCACTAGACA
AGAAAACGACGACGACCTTTGGGTAAGTAAAATTCAAGTTGATTCCTAAATTACAAACCT
TACTTTTTCATCTTTTTATACAAGTATAATAAATTGTATTTAAATTGAGATTAAGTAAAT
AACTGATAATTATTTACTTATTTTTTAACATTATGGCAGAATTTGGATGGGCTGCATGAG
GAAGAAGAAGCTTGTGTGAAC

Last Updated on 06.12.2018 16:11