Sequences from confirmation amplicon


Confirmation sequences for line 762C06 and hit At1g64190 are shown.
Line ID 762C06
Locus At1g64190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23827001
Primers used for PCR MS29 - 8474, show primer details
   
Sequence Name 39-K027065-022-762-C06C-8409
GenBank Accession LN503227 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K027065-022-762-C06C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAATCGCACT
ATCCCGAATCGCACTATCCCACTATCCCGAATATACTCATACTCATCATCGCACTATCCC
GAATCGCACTATCCCACTATCCTGAATATACTCATACTCATCATCGCACTATCCCGAATC
GGTCTCGCCGGACTCGCCGTGATGGGCCAAAACCTCGCCTTAAACATCGCCGAGAAAGGT
TTCCCAATCTCAGTCTACAATCGAACCACTTCCAAAGTCGACGAAACCCTAGATCGAGCC
GCCGTCGAAGGAAACCTCCCAGTCTCCGGCCAATACTCTCCCCGCGATTTCGTCCTCTCG
ATCCAACGGCCTAGATCTCTCATCA
   
Sequence Name 40-K027065-022-762-C06C-MS29
GenBank Accession LN503581 [GenBank]
Sequencing primer gene specific primer, MS29: 5'-GACGAGGATGATGAGAGATCTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K027065-022-762-C06C-ms29
CCGTTGGATCGAGAGGACGAAATCGCGGGGAGAGTATTGGCCGGAGACTGGGAGGTTTCC
TTCGACGGCGGCTCGATCTAGGGTTTCGTCGACTTTGGAAGTGGTTCGATTGTAGACTGA
GATTGGGAAACCTTTCTCGGCGATGTTTAAGGCGAGGTTTTGGCCCATCACGGCGAGTCC
GGCGAGACCGATTCGGGATAGTGCGATGATGAGTATGAGTATATTCAGGATAGTGGGATA
GTGCGATTCGGGATAGTGCGATGATGAGTATGAGTATATTCGGGATAGTGGGATAGTGCG
ATTCGGGATAGTGCGATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGAC

Last Updated on 06.12.2018 16:11