Sequences from confirmation amplicon


Confirmation sequences for line 290H06 and hit At1g66530 are shown.
Line ID 290H06
Locus At1g66530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24820970
Primers used for PCR 15fx - 8474, show primer details
   
Sequence Name 77-K111632-0022-290-H06C-15fx
GenBank Accession LN513570 [GenBank]
Sequencing primer gene specific primer, 15fx: 5'-AGGCATAGTTAAAACCACCATCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K111632-0022-290-H06C-15fx
TATCACACGAGCACCTTTACTTTCTTCACTAACCCCTTGCTGCTCAATTCCTCAATTACG
TTAGCGATATAAGGGTTGTAAAAGCTTTCTCCCTGTCATAAAACAATGTAGTTTCCATTA
CCCATTCATGTATATCTCAAATAATTTAGTATACGTCCCAGTTATTCCTTTACCTTTTCT
TCAAGCTCGATTCGAAGGCGTTTGTAAACCTTGGCAAACTCGTTTCGGCTGATTTCGCAA
ATCTTAGCCCATGCCTGGCGGTAAACAGGATCTCCACCCTGTACCGAAGAAAAAAATAGA
CAAGTAGGTGCATATTGAGTAGGTGCATATAGAAGAATATAAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATA
   
Sequence Name 61-K111631-0022-290-H06C-8409
GenBank Accession LN510556 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K111631-0022-290-H06C-8409
TTTTAATTCTTCTATATGCACCTACTCAATATGCACCTACTTGTCTATTTTTTTCTTCGG
TACAGGGTGGAGATCCTGTTTACCGCCAGGCATGGGCTAAGATTTGCGAAATCAGCCGAA
ACGAGTTTGCCAAGGTTTACAAACGCCTTCGAATCGAGCTTGAAGAAAAGGTAAAGGAAT
AACTGGGACGTATACTAAATTATTTGAGATATACATGAATGGGTAATGGAAACTACATTG
TTTTATGACAGGGAGAAAGCTTTTACAACCCTTATATCGCTAACGTAATTGAGGAATTGA
GCAGCAAGGGGTTAGTTGAAGAAAGTAAAGGTGCTCGTGTGATATTCATTGAAGGCTTTA
AAATCCCACTCATTGTTGTAAAGAGTGATGGTGGTTTTAACTATGCC

Last Updated on 06.12.2018 16:11