Sequences from confirmation amplicon


Confirmation sequences for line 088G03 and hit At1g68440 are shown.
Line ID 088G03
Locus At1g68440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25657997
Primers used for PCR 21nj - 8474, show primer details
   
Sequence Name 08-K114479-0022-088-G03C-oriL
GenBank Accession LN489829 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114479-0022-088-G03C-oriL
ATCTCTTTTTAGGCTTCCCCAGCCTTCGCATCCAAATCTCTCTTCTTTTTTTCTCTCTTT
CTCATAACAACAAAAAAACAAAAAAAAAACCTAATTAGTTTTTTCTTTCTGCAAAGATTA
TGGAGGTACCGGTGATTAATAGAATAAAAGATTTCGAAGTTGGTATAAACTCGATTAAGG
ATCCTTCGTTTCTTTCTCGATCTGTTGCTGTTTCCGGAATCGGAAAGCTACACCAAGCTT
ATGGTTTTTGGAAATGGGGAGCTTTGATTATCGCATTTCTAGCTAATTTCACCAACTTTG
TTAGTAAACTCAACAGTCTAGTTGTTAGGTTAAAAAAAATAAATGTCTCTGTTTCCTCTC
CAACTCTTTTTGATGATAACAATGGCGATCCCAATGTTTCTGGTTCCTCTACCGTCTCCC
CTGACAATAAAAAAAACAAAAAAAATAAGGCTGATAATAAAAATA
   
Sequence Name 24-K114480-0022-088-G03C-21nj
GenBank Accession LN497095 [GenBank]
Sequencing primer gene specific primer, 21nj: 5'-GAGTCAACATCCTCATCTTCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K114480-0022-088-G03C-21nj
GAAACATCGGAATCGCTATCGTAATCATCAAAAAGAGTTGGAGAGGAAACAGAGACATCT
ATTTTTCTTAACCTAACAACTAGACTGTTGAGTTTACTAACAAAGTTGGTGAAATAAGCT
AGAAATGCGATAATCAAAGCTCCCCATTTCCAAAAACCATAAGCTTGGTGTAGCTTTCCG
ATTCCGGAAACAGCAACAGATCGAGAAAGAAACGAAGGATCATTAATCGAGTTTATACCA
ACTTCGAAATCTCTTATTCTATTAATCACCGGTACCTCCATAATCTCTGCAGAAAGAAAA
AACTAATTAGGTTTTTTTTTTGTTTTTTTGTTGTTATGAGAAAGAGAGAAAAAAAGAAGA
GAGATTTGGATGCAAAGGCTTGGGGAACCCTTAAAAAAAGATACAATGGGTGTAATGCAT
GTCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAAAAAG
AGTAATTACCAATTTTTTTTCATTTCAAAAATGTAAATTTCCGCA

Last Updated on 06.12.2018 16:11