Sequences from confirmation amplicon


Confirmation sequences for line 374F09 and hit At1g68600 are shown.
Line ID 374F09
Locus At1g68600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25761894
Primers used for PCR 19eq - 8474, show primer details
   
Sequence Name 14-K116987-0022-374-F09C-19eq
GenBank Accession LN492681 [GenBank]
Sequencing primer gene specific primer, 19eq: 5'-AATACGAGAGAATACCGTCGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K116987-0022-374-F09C-19eq
AGATCCGCTGATTCAATCACAAGCCAAGAAGATTCTTTGGTATGTTTTACGCATCGTGGA
GACACATCTTTAAAATCCGGATTATGGTGATACCTATAGCATTAAAAGAGCTCTGTTTTC
GCAGCTTGATTTCGCTATATGGGAGCCTCCACATGGACCTTACAAAACGTTTAACCATCC
TTGGAAAAACTATGTCAAACTTAGTGGTGCAGTGAGGCATTGCGCGTTCACAGTCATGGC
AATGCATGGATGCATTCTTTCAGAAATACAGGTTCCTTGTTAAACTAATTCATTAAACAA
TTTGTTTTGTGTTATTAGTTATTACTCTGCTCCATTTTGCTACTAAGGTGAATGATTAAT
AGAAGAGTATACATAACTCGTTGAGTGTGTATCGTCATAAATGTCTAGCATGTGAAGAAA
AGAAAAGATGATGGCACATGAATTGAATAACCGGGGGAAAAAATGTCTAATCCAAAAGAA
AGAAAAAGAAAAAGAAATATATAGAGGAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGC
   
Sequence Name 02-K116986-0022-374-F09C-8409
GenBank Accession LN487204 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K116986-0022-374-F09C-8409
TTTCTTTTTCTTTTTCTTTCTTTTGGATTAGACATTTTTTCCCCCGGTTATTCAATTCAT
GTGCCATCATCTTTTCTTTTCTTCACATGCTAGACATTTATGACGATACACACTCAACGA
GTTATGTATACTCTTCTATTAATCATTCACCTTAGTAGCAAAATGGAGCAGAGTAATAAC
TAATAACACAAAACAAATTGTTTAATGAATTAGTTTAACAAGGAACCTGTATTTCTGAAA
GAATGCATCCATGCATTGCCATGACTGTGAACGCGCAATGCCTCACTGCACCACTAAGTT
TGACATAGTTTTTCCAAGGATGGTTAAACGTTTTGTAAGGTCCATGTGGAGGCTCCCATA
TAGCGAAATCAAGCTGCGAAAACAGAGCTCTTTTAATGCTATAGGTATCACCATAATCCG
GATTTTAAAGATGTGTCTCCACGATGCGTAAAACATACCAAAGAATCTTCTTGGCTTGTA
GATTGAACAGCGGATCTGTACCCGCTGTATAATGGATCATCTGAAGCTTGGTACGTGAGT
ATCTTCGACGG

Last Updated on 06.12.2018 16:11