Sequences from confirmation amplicon


Confirmation sequences for line 059D06 and hit At1g69770 are shown.
Line ID 059D06
Locus At1g69770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26251145
Primers used for PCR HP68 - 8474, show primer details
   
Sequence Name 65-K033553-022-059-D06C-8409
GenBank Accession LN511330 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K033553-022-059-D06C-8409
TGTTGACAGATTGTTGGTATTTCCTTCGGAGTCCCTAAAAAGTTATTGAAACGTGGACTT
TATTTGAAGGTATTGTTGCTCTGCTTGTACAAATTTCACATTACATCTCATGGTTTAGCC
TTGTTATATAGTTAATCATCTCTGGTGTTTTGATATAATAGGTAAGGTGGCTGAATTATG
ATGATTCTCATGATACATGGGAGCCTATTGAAGGACTCAGGTGAGCAAAACATTAAGAAT
GTTTGCATTAAAGTTATATATGTTAATGCGTTGTTAAAATCTCTAAATTTGTCCTTGAAC
AGTAATTGCCGGGGTAAAATTGAAGAGTTCGTTAAACTTGGATATAAATCTGGCATCCTT
CCGTTACCAGTATGTATTTTTTATACCTGATTCTTTTGCTTTCCTTAAACTTCTGAAGCT
AGTGCATAATTTTCAGATATTTAATCTAAAAGCCAATAATCTAAGTTATTGTATGTGATA
AGTTCTATGGGTCTTATGTTGTTCTGCGTCAGTTAATTGTTGAGGGGTGATAGATTTAGT
CACAGAACAGTTGATGTTTAGAGCAATATCCTGACATTGTTTATATGTTGTTGATGTTTA
GGGAGTGTTTTAATG
   
Sequence Name 65-K033553-022-059-D06C-HP68
GenBank Accession LN511331 [GenBank]
Sequencing primer gene specific primer, HP68: 5'-AACATCAACACCTCCCTAAACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K033553-022-059-D06C-HP68
GCTCTAACTCAACTGTTCTGTGACTAAATCTATCACCCCTCAACAATTAACTGACGCAGA
ACAACATAAGACCCATAGAACTTATCACATACAATAACTTAGATTATTGGCTTTTAGATT
AAATATCTGAAAATTATGCACTAGCTTCAGAAGTTTAAGGAAAGCAAAAGAATCAGGTAT
AAAAAATACATACTGGTAACGGAAGGATGCCAGATTTATATCCAAGTTTAACGAACTCTT
CAATTTTACCCCGGCAATTACTGTTCAAGGACAAATTTAGAGATTTTAACAACGCATTAA
CATATATAACTTTAATGCAAACATTCTTAATGTTTTGCTCACCTGAGTCCTTCAATAGGC
TCCCATGTATCATGAGAATCATCATAATTCAGCCACCTTACCTATTATATCAAAACACCA
GAGATGATTAACTATATAACAAGGCTAAACCATGAGATGTAATGTGAAATTTGTACAAGC
AGAGCAACAATACCTTCAAATAAAGTCCACGTTTCAATAACTTTTTAGGGACTCCGAAGG
AAATACCAACAATCTTGTCAACAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11