Sequences from confirmation amplicon


Confirmation sequences for line 402B10 and hit At1g70710 are shown.
Line ID 402B10
Locus At1g70710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26662301
Primers used for PCR 12tq - 8474, show primer details
   
Sequence Name 21-K116987-0022-402-B10C-12tq
GenBank Accession LN495827 [GenBank]
Sequencing primer gene specific primer, 12tq: 5'-ACGAATCATAGAAATGAACCAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K116987-0022-402-B10C-12tq
ATGCATATGGACACGAATCTCTTATCTCACAAGCAAAGAATAATTGATTAAAAATTAAAA
TCTTTTTGACTATTAAATTTGACTATTAAAGAAAAACAAAGAGAATATTAAAATGTTTGT
AATAATTAAAATTAATTACCACAGTTAATGCTAATGGTTAAAGTGTGAAAACAGAGTAAC
GTACCCTAGTGGCACGGTCAAGTAGAAGTCTGGAATGAGCAATTGTCATGAGCAATTTCG
CGAGGGTTTCCGAGATGGTGATTGCTGCTTATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 09-K116986-0022-402-B10C-8409
GenBank Accession LN490363 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K116986-0022-402-B10C-8409
CAGCAATCACCATCTCGGAAACCCTCGCGAAATTGCTCATGACAATTGCTCATTCCAGAC
TTCTACTTGACCGTGCCACTAGGGTACGTTACTCTGTTTTCACACTTTAACCATTAGCAT
TAACTGTGGTAATTAATTTTAATTATTACAAACATTTTAATATTCTCTTTGTTTTTCTTT
AATAGTCAAATTTAATAGTCAAAAAGATTTTAATTTTTAATCAATTATTCTTTGCTTGTG
AGATAAGAGATTCGTGTCCATATGCATTTATAATAATTGTTTTATTTCAGTACTTTTTTG
GGTGATGGTTGGTTCATTTAAATGATTCG

Last Updated on 06.12.2018 16:11