Sequences from confirmation amplicon


Confirmation sequences for line 203G06 and hit At1g71040 are shown.
Line ID 203G06
Locus At1g71040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26797714
Primers used for PCR 17sm - 8474, show primer details
   
Sequence Name 49-K116549-0022-203-G06C-17sm
GenBank Accession LN506959 [GenBank]
Sequencing primer gene specific primer, 17sm: 5'-GAAACCAGTATCGACCAAATCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K116549-0022-203-G06C-17sm
TTTCAAGTCAACATCGAAAACGGCAATTCTTGCCAACAATGCACCTTATCCTTACCCGTC
GGGAGATCCCGTCACGGAAGAGAACAGCAAAGTCATGAAGTTTATAATCAACTACAAATC
AGAAGTTGACACGTCGATTATTCCGAAGAAGCTAATTGAATACCCTCCTGCTCATGTATC
AACCTCCACACGTACACGTTACATTGCTATGTTCGAGTATGTGTCAAGCATTGACGAGCC
AACACATCTATACATCAACGGTCTGCCTTACAACGCTCCCGTAACCGAAACTCCAAAAAT
CGGCACAAGCGAGGTATTTATAACCAAAATTTCACCTATCGAGTCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 09-K116548-0022-203-G06C-8409
GenBank Accession LN490355 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K116548-0022-203-G06C-8409
GGTGAAATTTTGGTTATAATACCTCGCTTGTGCCGATTTTTGGAGTTTCGGTTACGGGAG
CGTTGTAAGGCAGACCGTTGATGTATAGATGTGTTGGCTCGTCAATGCTTGACACATACT
CGAACATAGCAATGTAACGTGTACGTGTGGAGGTTGATACATGAGCAGGAGGGTATTCAA
TTAGCTTCTTCGGAATAATCGACGTGTCAACTTCTGATTTGTAGTTGATTATAAACTTCA
TGACTTTGCTGTTCTCTTCCGTGACGGGATCTCCCGACGGGTAAGGATAAGGTGCATTGT
TGGCAAGAATTGCCGTTTTCGATGTTGACTTTGAAAAGTCAACGAGTACGTCGACAATCT
CCGACGGAGCTAGGAGAACAGATTTGGT

Last Updated on 06.12.2018 16:11