Sequences from confirmation amplicon


Confirmation sequences for line 972C01 and hit At1g71270 are shown.
Line ID 972C01
Locus At1g71270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26867034
Primers used for PCR 36ke - 8474, show primer details
   
Sequence Name 44-K113531-0022-972-C01C-36ke
GenBank Accession LN505249 [GenBank]
Sequencing primer gene specific primer, 36ke: 5'-AAAAGGGGAAAAATCAACAGAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K113531-0022-972-C01C-36ke
GCTCCGAAGAGCACTTCATAAGGATATTTGAGAGAGCTGGCTTCGGCTATGTGCGGGATC
AAAGCAGGTTGATCAATATCCTACAGCGGTCATACTGAAACTTAAGTCTTTTAACGAATA
TTGTTAAACCAAACAAACTTTGGATTTACTTCTTCTATAGCAAAAAAAAACAAATTGATA
CCACACTAATCCAAATTGAAAATGAGAAAAACAAATCGAAACTTCCATCTATTTGAGGAG
CCTTTAAAATTAAGGATACATATCGTAAAAACCAAAACTGAAATGAACCAAAATATTTGA
TTCCTATAATTTATAACCTAAGACTAAAATATCTGAACCAAAACCAAAGTGGGCACTCCT
CAATAAAACTTATGGTGTACAATACATTAATATAACTTATACTTTATTGTCCAGAAGCTT
ATAGCCCTGAGGAAACCCAAAACAAACATTCAGATTCTTCAACAGAGTGTGTTATTAAAG
TACAAGTGAGCATTCTAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 19-K113530-0022-972-C01C-8409
GenBank Accession LN494840 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K113530-0022-972-C01C-8409
TCATTGAAATTTAGAATGCTCACTTGTACTTTAATAACACACTCTGTTGAAGAATCTGAA
TGTTTGTTTTGGGTTTCCTCAGGGCTATAAGCTTCTGGACAATAAAGTATAAGTTATATT
AATGTATTGTACACCATAAGTTTTATTGAGGAGTGCCCACTTTGGTTTTGGTTCAGATAT
TTTAGTCTTAGGTTATAAATTATAGGAATCAAATATTTTGGTTCATTTCAGTTTTGGTTT
TTACGATATGTATCCTTAATTTTAAAGGCTCCTCAAATAGATGGAAGTTTCGATTTGTTT
TTCTCATTTTCAATTTGGATTAGTGTGGTATCAATTTGTTTTTTTTTGCTATAGAAGAAG
TAAATCCAAAGTTTGTTTGGTTTAACAATATTCGTTAAAAGACTTAAGTTTCAGTATGAC
CGCTGTAGGATATTGATCAACCTGCTTTGATCCCGCACATAGCCAAAGCCAGCTCTCTCA
AATATCCTTATGAAGTGCTCTTCAGGAGCCTGCACAAGCTACTTATGGATACAGCTACTT
CTGAGTTCTGTTGATTTTCCCCCTT

Last Updated on 06.12.2018 16:11