Sequences from confirmation amplicon


Confirmation sequences for line 366F03 and hit At1g77710 are shown.
Line ID 366F03
Locus At1g77710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29208032
Primers used for PCR HM45 - 8474, show primer details
   
Sequence Name 01-K032516-022-366-F03C-8409
GenBank Accession LN486460 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K032516-022-366-F03C-8409
TTACAATTGAATATATCCTGACATATAACTTGACCAAATTCAACTCACTTCAATTGTGAC
GATTACATATTTATTCAACGAAGTTGTACATAAGCATTTCAGTATATTATTATGTTTTTT
TTGGAACTGAGTTTTTAATCATTGCTTACACATCATCTATGGATCCATCACAAACACAGC
TCCAACTCTATCACGCGGGATCAATCTTAGTTCAGATCCGTGCTTCAGAAAAACGTTTCC
TGACATCATTCACAACACCACATTCATTAACAAGGAGCTTGATTTCTACTTGTATGTTGC
TAATGACCACACCATAAAAAAGACAGCTGAGATATTACAGCGGAACCCGACTGTGTGTAA
ATAATCTAAAGGTTGCTTTCCTATGGACACTGCACTGAAGTCACTTAGAGGCTAAGGAAC
ACCAAAAAAGAAAAGCACGCAACCATTAAGGACAGTACTAGTGATCTTCTAAGACTCTAT
TAGTACATTGGCAATCAAAGCATGCAAGAGAGAAACAAAACTGAGAATTGCAATGGCATA
AAAGATAGAGAATTGAGTAAAACGCAATAACCAAGTGTTTAGCATTGTGATAGTTACCTG
CACTCTGTTGAGGATTGATCCCGATCCCATCTGCACACATGAATACAGATACATCATCAC
ATCAGAAATCTTGAGAATTGATGAATATGGATTACATCAGCCGAAACTAATAATCAATAG
ATGACTAAACACACCCAAAATATCATAGATTAGTTATGAGAACTATAATTCTATTCGAAA
CAAATCCCTTTAAGATTTTCCAGCACCAGAAAGCTTTAAACTTGTAAACTTTGTAGATAC
ATAAAGAGCTCAAGGAAAAGATTTACATGATTCATGTCTAAGATAACATTCTCGTGAATG
CANCAACATATGAAGATGAAATGAAATCAATTTGAAAGGACACATTGTCCTGCATATGTC
CAT
   
Sequence Name 01-K032516-022-366-F03C-HM45
GenBank Accession LN486461 [GenBank]
Sequencing primer gene specific primer, HM45: 5'-TGTGAGTTTGTACGATAAATGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K032516-022-366-F03C-HM45
CTTTAATTTGATTCATTTCATCTTCATATGTTTGTGCATTCACGAGAATGTTATCTTAGA
CATGAATCATGTAAATCTTTTCCTTGAGCTCTTTATGTATCTACAAAGTTTACAAGTTTA
AAGCTTTCTGGTGCTGGAAAATCTTAAAGGGATTTGTTTCGAATAGAATTATAGTTCTCA
TAACTAATCTATGATATTTTGGGTGTGTTTAGTCATCTATTGATTATTAGTTTCGGCTGA
TGTAATCCATATTCATCAATTCTCAAGATTTCTGATGTGATGATGTATCTGTATTCATGT
GTGCAGATGGGATCGGGATCAATCCTCAACAGAGTGCAGGTAACTATCACAATGCTAAAC
ACTTGGTTATTGCGTTTTACTCAATTCTCTATCTTTTATGCCATTGCAATTCTCAGTTTT
GTTTCTCTCTTGCATGCTTTGATTGCCAATGTACTAATAGAGTCTTAGAAGATCACTAGT
ACTGTCCTTAATGGTTGCGTGCTTTTCTTTTTTGGTGTTCCTTAGCCTCTAAGTGACTTC
AGTGCAGTGTCCATAGGAAAGCAACCTTTAGATTATTTACACACAGTCGGGTTCCGCTGT
AATATCTCAGCTGTCTTTTTTATGGTGTGGTCATTAGCAACATACAAGTAGAAATCAAGC
TCCTTGTTAATGAATGTGGTGTTGTGAATGATGTCAGGAAACGTTTTTCTGAAGCACGGA
TCTGAACTAAGATTGATCCCGCGTGATAGAGTTGGAGCTGTGTTTGTGATGGATCCATAG
ATGATGTGTAAGCAATGATTAAAAACTCAGTTCCAAAAAAAACATAATAATATACTGAAA
TGCTTATGTACAACTTCGTTGAATAAATATGTAATCGTCACAATTGAAGTGAGTTGAATT
TGGNNTCAGTATATGTCAGGATATATTCAATTTGTAATGGCTTCATGTCCGGNAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTATTACCATTTT
TTTTTCATTCAAAATGT

Last Updated on 06.12.2018 16:11