Sequences from confirmation amplicon


Confirmation sequences for line 107A03 and hit At1g78240 are shown.
Line ID 107A03
Locus At1g78240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29433499
Primers used for PCR UF27 - 8474, show primer details
   
Sequence Name 15-K030219-022-107-A03C-8409
GenBank Accession LN492847 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K030219-022-107-A03C-8409
CATTTACAATTGAATATATCCTGAATGTCTGCATGTACCAGGTCATATGTTCTCGGGTAA
GTCGGGAATGGTTCACACCTGAAGAAAAAGAGATGTTCAGAAACACTTCTATGGCTAAAA
TGAAAATCCAATAAGGCTTTTGAACTGACCAGTTGTGCAAAACTCCGACAAAGCCACGGT
CAAGTATCATGGGGAGGTGGTTAGGTCCAGCTGTAGGGACCACATTCATGACCCAGACTG
ATTTTCTTGCTTCCAACAAGGCGGAATTGAGACCACCGAATTGAGCATTCATATCCAGTA
CGTTTCTGAGCATGTTATAAGGCGGTGATGGATCTTCATCGCCGGGTCTTTTTGGATGAT
CAGAGAATATCAGAGGAGACAAGAGAGACCAGTATTCTCTCACTGTTATCTTCCAGTTCT
CAGCATCCAAAAAGACCCGGCGATGAAGATCCATCACCGCCTTATAACATGCTCAGAAAC
GTACTGGATATGAATGCTCAATTCGGTGGTCTCAATTCCGCCTTGTTGGAAGCAAGAAAA
TCAGTCTGGGTCATGAATGTGGTCCCTACAGCTGGACCTAACCACCTCCCCATGATACTT
GACCGTGGCTTTGTCGGAGTTTTGGCACAACTGGTCAGTTCAAAAGCCTTATTGGATTTT
CATTTTAGCCATAGAAGTG
   
Sequence Name 16-K030219-022-107-A03C-UF27
GenBank Accession LN493314 [GenBank]
Sequencing primer gene specific primer, UF27: 5'-GGATGCTGAGAACTGGAAGATAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K030219-022-107-A03C-UF27
TCTGATATTCTCTGATCATCCAAAAAGACCCGGCGATGAAGATCCATCACCNGCCTTATA
ACATGCTCAGAAACGTACTGGATATGAATGCTCAATTCGGTGGTCTCAATTCCGCCTTGT
TGGAAGCAAGAAAATCAGTCTGGGTCATGAATGTGGTCCCTACAGCTGGACCTAACCACC
TCCCCATGATACTTGACCGTGGCTTTGTCGGAGTTTTGCACAACTGGTCAGTTCAAAAGC
CTTATTGGATTTTCATTTTAGCCATAGAAGTGTTTCTGAACATCTCTTTTTCTTCAGGTG
TGAACCATTCCCGACTTACCCGAGAACATATGACCTGGTACATGCAGACATTCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACATT

Last Updated on 06.12.2018 16:11