Sequences from confirmation amplicon


Confirmation sequences for line 605B06 and hit At1g79000 are shown.
Line ID 605B06
Locus At1g79000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29717872
Primers used for PCR IF12 - 8474, show primer details
   
Sequence Name 26-K032519-022-605-B06C-IF12
GenBank Accession LN497808 [GenBank]
Sequencing primer gene specific primer, IF12: 5'-GCTGCATACTGATGGTATCTGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K032519-022-605-B06C-IF12
TTCCGATTTGTGACAAGTAAGAATTCTTTCGAGGTCTTTTTGCTCTTTGATTTTATAATC
CCTTCTTTGTTGTATTGCACATAAGATTATGTAGAAGTCCCGAGAGATACATTTATTGGA
TGGGCTATGTGTCCTTGTTACTGATTAAGTTCCTCTGAATCTAAAATGTCATTCTTCTTT
TTAGGTGTTACGAGGCTGAGCAGAGACGTGAAGACAGGGAAAGGCATCCTGTTAATTTTA
AGGATAAGCATGCGTTGTATCCAGTGAGTTGCATTTTCTTGTTGAGGATAGTGTTCTTTA
GCTTTGTCTATGATCAGTTTTGTGAATATTGGTGGTATTGATATCGCAGGTTGAGATTAT
GGATATTCCTGCAATACAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
C
   
Sequence Name 26-K032519-022-605-B06C-8409
GenBank Accession LN497807 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K032519-022-605-B06C-8409
AATCTCAACCTGCGATATCAATACCACCAATATTCACAAAACTGATCATAGACAAAGCTA
AAGAACACTATCCTCAACAAGAAAATGCAACTCACTGGATACAACGCATGCTTATCCTTA
AAATTAACAGGATGCCTTTCCCTGTCTTCACGTCTCTGCTCAGCCTCGTAACACCTAAAA
AGAAGAATGACATTTTAGATTCAGAGGAACTTAATCAGTAACAAGGACACATAGCCCATC
CAATAAATGTATCTCTCGGGACTTCTACATAATCTTATGTGCAATACAACAAAGAAGGGA
TTATAAAATCAAAGAGCAAAAAGACCTCGAAAGAATTCTTACTTGTCACAAATCTGGAAG
TGTTTGCACTGGCTGCAGACCCAACGATTACCAGATACCATCAGTATGCA

Last Updated on 06.12.2018 16:11