Sequences from confirmation amplicon


Confirmation sequences for line 071G06 and hit At1g80560 are shown.
Line ID 071G06
Locus At1g80560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30289891
Primers used for PCR FV19 - 8474, show primer details
   
Sequence Name 71-K034063-022-071-G06C-FV19
GenBank Accession LN512502 [GenBank]
Sequencing primer gene specific primer, FV19: 5'-TCTGCTAGTCTCAGTGATTCGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K034063-022-071-G06C-FV19
TTTAGTATGCAAGCAGTATGATCTAGTCTGAACATAAACCCGCCATGTGTAATACAGGGA
CCTGGACTCTTTGAACCTATACATGGTTCTGCACCTGATATTGCTGGACAGGTTCTAACT
CTATCCTTCAAACAAACACGGTTTTTGTTCCCTGATATCAAAACTAGTTTAATGCCAAAA
TCAAAGAGCACAACTTGTTGATTCTTTGAATTTTCAGGATAAGGCAAACCCATTGGCAAC
CATTCTAAGCGCTGCAATGCTTCTGAAATACGGACTTGGAGAAGAAAAGGCAGCCAAGAG
AATTGAAGACGCAGTGTTGGTTGCTCTGAACAACGGATTCAGAACTGGAGAAAAAGAAAG
AGTAATTACCAATTTTCTACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACATTCAC
   
Sequence Name 04-K034063-022-071-G06C-8409
GenBank Accession LN487817 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K034063-022-071-G06C-8409
AAGCCATGTAGAAAATTGGTAATTACTCTTTCTTTTTCTCCAGTTCTGAATCCGTTGTTC
AGAGCAACCAACACTGCGTCTTCAATTCTCTTGGCTGCCTTTTCTTCTCCAAGTCCGTAT
TTCAGAAGCATTGCAGCGCTTAGAATGGTTGCCAATGGGTTTGCCTTATCCTGAAAATTC
AAAGAATCAACAAGTTGTGCTCTTTGATTTTGGCATTAAACTAGTTTTGATATCAGGGAA
CAAAAACCGTGTTTGTTTGAAGGATAGAGTTAGAACCTGTCCAGCAATATCAGGTGCAGA
ACCATGTATAGGTTCAAAGAGTCCAGGTCCCTGTATTACACATGGCGGGTTTATGTTCAG
ACTAGATCATACTGCTTGCATACTAAAAGATCATATTATTTTATATCATACCGAATCACT
GAAACCTAGCAG

Last Updated on 06.12.2018 16:11