Sequences from confirmation amplicon


Confirmation sequences for line 010F09 and hit At2g01490 are shown.
Line ID 010F09
Locus At2g01490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:222213
Primers used for PCR DM17 - 8474, show primer details
   
Sequence Name 66-K101747-0022-010-F09C-8409
GenBank Accession LN511568 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K101747-0022-010-F09C-8409
CATTTACATTGGTAGCCATTTATTTTTTCTCTACAACAATTTTAACATTCGTGATCCAAC
GAAAAGTATGTAATAAGCGAGCCAAAAGAAAGAAAAAGATACCATGCCCAACTTTGTTTA
TAGAGAGTTGTTTGGGTTGCTTCAATTTCCCATCATCACCAAAAGCTTTTTCTGAGATTC
AAACACATGGAACCTTTAGCTTATAGCTGGAGTAGAACAAAAGCACACTAGAACATGAAT
CAAGGATATAGAATTAGTCTAGACAATTGCATCCTAATCAAAGGATGACCGAAGCTTCCC
ACACATAAAAAAAAGTGCCAAAACATTGCACTAAGATTCATATTCATATTCCATTGTAGT
AAACAAAAGAAATAGTTATAAACAAAGCACTTGCACACAAACATGAACATAAAACCGGTA
AAAACATACTCATTTGGATTCACTACCAAACAATTGTTTGGATTATATCTNAACAGAATC
AAGAATCTAAGCAATTGAAGAAAAGTAGGAATATTGTTACCTTCAAAGAAGAAGGAGATT
TTCTCAGCGCTCTCAAGAAA
   
Sequence Name 66-K101753-0022-010-F09C-DM17
GenBank Accession LN511569 [GenBank]
Sequencing primer gene specific primer, DM17: 5'-AATTACTTCTTTGAGAGCGCTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K101753-0022-010-F09C-DM17
GTACATATTCCTACTTTTCTTCATTGCTTAGATTCTTGATTCTGTTTAGATATAATCCAA
ACAATTGTTTGGTAGTGAATCCAAATGAGTATGTTTTTACCGGTTTTATGTTCATGTTTG
TGTGCAAGTGCTTTGTTTATAACTATTTCTTTTGTTTACTACAATGGAATATGAATATGA
ATCTTAGTGCAATGTTTTGGCACTTTTTTTTATGTGTGGGAAGCTTCGGTCATCCTTTGA
TTAGGATGCAATTGTCTAGACTAATTCTATATCCTTGATTCATGTTCTAGTGTGCTTTTG
TTCTACTCCAGCTATAAGCTAAAGGTTCCATGTGTTTGAATCTCAGAAAAAGCTTTTGGT
GATGATGGGAAATTGAAGCAACCCAAACAACTCTCTATAAACAAAGTTGGGCATGGTATC
TTTTTCTTTCTTTTGGCTCGCTTATTACATACTTTTCGTTGGATCACGAATGTTAAAATT
GTTTGTAGAGAAAAAATAAATGGCTACCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATAT

Last Updated on 06.12.2018 16:11