Sequences from confirmation amplicon


Confirmation sequences for line 209A01 and hit At2g02710 are shown.
Line ID 209A01
Locus At2g02710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:758976
Primers used for PCR DB21 - 8474, show primer details
   
Sequence Name 14-K029909-022-209-A01C-8409
GenBank Accession LN492379 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K029909-022-209-A01C-8409
TTCATTGAATATATCCTGTACTAATTACTTTCAGTTCTACATGTCATATTCNGGGTTTTG
TATCAAGGCCAAAAAATGAAAGACAAAAGGATTTGGTAACAGGAAGTATGTAGTCATGAA
GGGATATGTACCTTGCCTGAAGCATTACGAACTGGTGAGATGTGAAGAAGATTCCAAAAT
GAGCTCTTATCTTTTCTGTTACTGATAAAAAACATATATGATCAGCCCAAAACAAATCAA
AGAAACAAGATTCGATCGCAAAAATGTTTTGAAGAAGTAAATCTTCAACCTGTAATTTAG
TATTTGCACTGTGCATGATTGTCCCTTTAAGATGCATTCCTTCATCTGAACCAAATAAAA
CATTCACAATTATCAATCATGCCATATACTAATTAACAAGTTAAAACGCAATCTCTACCT
CGTATAGTACTGAGGAATCAGTATCAACCCCACTCAGAAATCTACAGTTTTGTCCCAACA
CTTCCTGTCTCTTGTAACCTACAAACATTGATATAAATAAGAACTGTGACCAAAATCTAA
TAACGGTAACTTTATTTCTTCAAACCTAGACACACTTACCGGTCAGTGTCAAAAAGGCAT
CACTCGCATAAATGATAGGCATGTCTGGTAAGCATGGATTGGTTCTGCAAAGTTACGAGA
ACAATGTCAATATCAAAATCTATTGTAGCAGACTGTAACTCCATAACACAATAAAAAAAT
AATGTACTCACAATACGAAACTTTGTTTGATTCTACCGAGAGAAATAACTACGACGAGCT
TAGACAATCTACTCCTCGCAAGCAGTACCGCTTTCCGCAGACTAATCTACCACTCAC
   
Sequence Name 14-K029909-022-209-A01C-DB21
GenBank Accession LN492380 [GenBank]
Sequencing primer gene specific primer, DB21: 5'-CTTAGTGCATTACAGCGAGTTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K029909-022-209-A01C-DB21
TAGATTGTCTAAGCTCGTCGTTAGTTATTTCTCTCGGTAGAATCAAACAAAGTTTCGTAT
TGTGAGTACATTATTTTTTTATTGTGTTATGGAGTTACAGTCTGCTACAATAGATTTTGA
TATTGACATTGTTCTCGTAACTTTGCAGAACCAATCCATGCTTACCAGACATGCCTATCA
TTTATGCGAGTGATGCCTTTTTGACACTGACCGGTAAGTGTGTCTAGGTTTGAAGAAATA
AAGTTACCGTTATTAGATTTTGGTCACAGTTCTTATTTATATCAATGTTTGTAGGTTACA
AGAGACAGGAAGTGTTGGGACAAAACTGTAGATTTCTGAGTGGGGTTGATACTGATTCCT
CAGTACTATACGAGGTAGAGATTGCGTTTTAACTTGTTAATTAGTATATGGCATGATTGA
TAATTGTGAATGTTTTATTTGGTTCAGATGAAGGAATGCATCTTAAAGGGACAATCATGC
ACAGTGCAAATACTAAATTACAGGTTGAAGATTTACTTCTTCAAAACATTTTTGCGATCG
AATCTTGTTTCTTTGATTTGTTTTGGGCTGATCATATATGTTTTTTATCAGTAACAGAAA
AGATAAGAGCTCATTTTGGAATCTTCTTCACATCTCACCAGTTCGTAATGCTTCAGGCAA
GGTACATATCCCTTCATGACTACATACTTCCTGTTACCAAATCCTTTTGTCTTTCATTTT
TTGGCCTTGATACAAAACCCGAATATGACATGTAGAACTGAAAGTAATTAGTACAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAA

Last Updated on 06.12.2018 16:11