Sequences from confirmation amplicon


Confirmation sequences for line 556F03 and hit At2g03090 are shown.
Line ID 556F03
Locus At2g03090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:918661
Primers used for PCR JL43 - 8474, show primer details
   
Sequence Name 25-K101383-0022-556-F03C-8409
GenBank Accession LN497373 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K101383-0022-556-F03C-8409
CCATTTACATTGAATATATCCTGCAACCATCAACCATCAACCTTCTTCACTCCCATCAAA
GCAAAACTATCTTTCTCCTTCTCATTCCTTTTTCTCTCACTCTCCTCCATTAAAGCTCTG
CACTTTCTCAAAGAGAATGTTCATGTAATATAACATCTTCCTTTCAAAGTCTTTCTTTTA
CTGTTTTTTTTCTTCTATCTTTCTAATAACCAATAAAGGTATAATTTTTGTTTGAAATTT
GTTCTATTGCAGGGGTAAGATGGGTCTTTTGGGAATTGCTCTGTTTTGTTTTGCTGCAAT
GGTGTGCTCTGTTCATGGCTATGACGCTGGATGGGTCAATGCTCATGCTACCTTCTATGG
TGGAAGTGATGCTTCAGGAACAATGGGTATGTGCTTCTCACTTTGTTCTCTAAAATGTCT
CAAAGAAAACGAANATCTAGGATATTTACAATCTTGTTGATGTTGTTG
   
Sequence Name 25-K101389-0022-556-F03C-JL43
GenBank Accession LN497374 [GenBank]
Sequencing primer gene specific primer, JL43: 5'-CTCCACCTAGAGACAACAACATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K101389-0022-556-F03C-JL43
TTTCGTTTTCCTGAGACATTTTAGAGAACAAAGTGAGAAGCACATACCCATTGTTCCTGA
AGCATCACTTCCACCATAGAAGGTAGCATGAGCATTGACCCATCCAGCGTCATAGCCATG
AACAGAGCACACCATTGCAGCAAAACAAAACAGAGCAATTCCCAAAAGACCCATCTTACC
CCTGCAATAGAACAAATTTCAAACAAAAATTATACCTTTATTGGTTATTAGAAAGATAGA
AGAAAAAAAACAGTAAAAGAAAGACTTTGAAAGGAAGATGTTATATTACATGAACATTCT
CTTTGAGAAAGTGCAGAGCTTTAATGGAGGAGAGTGAGAGAAAAAGGAATGAGAAGGAGA
AAGATAGTTTTGCTTTGATGGGAGTGAAGAAGGTTGATGGTTGATGGTTGCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCA

Last Updated on 06.12.2018 16:11