Sequences from confirmation amplicon


Confirmation sequences for line 659D12 and hit At2g11270 are shown.
Line ID 659D12
Locus At2g11270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:4484803
Primers used for PCR 17ou - 8474, show primer details
   
Sequence Name 66-K115121-0022-659-D12C-17ou
GenBank Accession LN511673 [GenBank]
Sequencing primer gene specific primer, 17ou: 5'-AAGTGTGCCAGTGATTACTTGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K115121-0022-659-D12C-17ou
GAGTTGGAGTCATAAACAGTTAAGAGGTAGAAGAAAGTTTGAGAAATAAGTGACCATTGG
ATACAACTTTTTCTGTTTGAAATGTTATTGTGACCGAGGGACAAATCTTTTGAGGTGTAG
AAACAACAGAGTCGATTAAAGAGTTATAGTGATTAATGCTTTGCGTAGTTGTCTTTAGGA
AATGTTCTTGATCCTCCTCTCTCTCTCAAAAAAGATGGGTCAATGTTCTGTTTCTGCGTT
TCGTCCATATACTTCAGGAAATGGTATTCAGACATTTGATCATTCCCTTTAACTTAAACA
ACAATAGGGCTATTACACCAATTCACTCTTAAAAATACTCATCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCAC
   
Sequence Name 18-K115120-0022-659-D12C-8409
GenBank Accession LN494427 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115120-0022-659-D12C-8409
TCGGACATGAGCCTTTACATTGATGAGTATTTTTAAGAGTGAATTGGTGTAATAGCCCTA
TTGTTGTTTAAGTTAAAGGGAATGATCAAATGTCTGAATACCATTTCCTGAAGTATATGG
ACGAAACGCAGAAACAGAACATTGACCCATCTTTTTTGAGAGAGAGAGGAGGATCAAGAA
CATTTCCTAAAGACAACTACGCAAAGCATTAATCACTATAACTCTTTAATCGACTCTGTT
GTTTCTACACCTCAAAAGATTTGTCCCTCGGTCACAATAACATTTCAAACAGAAAAAGTT
GTATCCAATGGTCACTTATTTCTCAAACTTTCTTCTACCTCTTAACTGTTTATGACTCCT
AACTCTTATGTTTAATTACTGCGAATTCAAGGAAACATCANGTAATCACTGGCACAC

Last Updated on 06.12.2018 16:11