Sequences from confirmation amplicon


Confirmation sequences for line 261F08 and hit At2g14700 are shown.
Line ID 261F08
Locus At2g14700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6294512
Primers used for PCR CQ06 - 8474, show primer details
   
Sequence Name 66-K105568-0022-261-F08C-CQ06
GenBank Accession LN511613 [GenBank]
Sequencing primer gene specific primer, CQ06: 5'-TGCAAAATTAGACAAGGAGAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K105568-0022-261-F08C-CQ06
TATAATATTGTGATGGGCTTAAAGTAGATTTAGCTTTGTGAGATTAAGATTAATGTACCC
TAAATCCTATATATATATATATGTAGCCACAAAGATAAATAACACAATAACACAGAATTT
TCAGTATCTAAACTTAAATTCTGACATGGTATCAGAGCTTTAGGTTTTTTAGGTTTACAA
GTTTTAAGGTTTTTGTTTTGATTCCACTAATATGAAAAAAAAAAAAATTGTTTTTATTTT
TTAATTTTTGTTAGCCTTTAGGGTTTGTTGTGGGGTTTTGTTCTTGTAAATCTTGGGGAG
TTTTTTTGGGGTTCCCCTTTTGAGGTTAAAAATTTGTTTTTTTTTTCCAAGCTTTTTTTC
AAGTTTGGAAAAATTCCCTTGGGGAAAAAAAAAAGGGTTTTAAAAAAAGGGGTTTAAATA
ATAATAATTTTTTTTTTTTTTTTGGAAAAAGGGGGTTTAAAACCTCTTTACGGGGGGGGA
ACCAGGGGTTTTAGGGGTTTTTGGGTTAAAAAAAAACCATTCCTTTTTGGGTTTTAGGGG
GTTTAAAAGGGTTTTTTGCCGGGAAATCCCCCGGGGACCCCCAGGGATTAGGGGGGCCAA
TGGGGAAAAAAAAAAAAAAAATTCCCCTTTTTTTTTTCATTCAAAAAGGGAAAAG
   
Sequence Name 82-K105569w-0022-261-F08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K105569w-0022-261-F08C-8409
TCGGAAAACACTCTAAACACCTAAAAACCAAAAACGAACTGACTCATACTAAACCAAAAA
ACCCTAAAACACCTAGATCAACCGCCGTAAAGCAGATCTAAACACCACTCTTCCAAAAAA
AATAAAAATAATAATAATAATCAAAACCCTAATCTTAACAACACTCTTTCTCTCACCAAA
GGAAATCTTACAAACTTGAAACAAAGACTCGAAAAAAAAAAACAAATCTTAAACATCAAA
AGGGAAACCCAAAATAAACTCCCAAAAATTAACAAAAACAAAACCCAACAACAAACCCTA
ACGACTAAAAAAACTTAAAAACTAAAAACAATTTTTTTTTTTTCCATTTAGGGGAAACAA
AAAAAAAACCCTAAAAATTTGAAACCCAAAAAACCCAAAGCCCCTAAACCCTGGTAAAAT
TTTAATTTAAAAACCGAAAATTTCGGGGTAATGGGGTAATTAACCTTGGGGGCCCCATAA
ATTTTTTTAGGGATTTGGGGGAAATTTATTTTAATCCCCAAAAAGTAAATTTTTTTTAAC
CCCCCCCAAAATTTTATGGGGCGGGGGCCCCCCCTTTTCCAAAAAAATTTCTTTCCCCCT
TGCAAAATTTGAAAAA

Last Updated on 06.12.2018 16:11