Sequences from confirmation amplicon


Confirmation sequences for line 159C01 and hit At2g16365 are shown.
Line ID 159C01
Locus At2g16365 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7077543
Primers used for PCR 67pq - 3144, show primer details
   
Sequence Name 45-K115187-0022-159-C01C-67pq
GenBank Accession LN505632 [GenBank]
Sequencing primer gene specific primer, 67pq: 5'-CAATAAGATTTGATTTTGCTCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K115187-0022-159-C01C-67pq
TTATTATTCTAAAACATAACTAAAGTAAAATCTTATATATAGATAAATTGAAGAAAGAGA
TTTTTACTATGTTAAGATTAAGATTAAGGGAAATGAAGACGTCTGTCATGATAAATTTTT
ACTTTTCTCCATTTCACAAATATTTAAATTTTTACCCGAACACTGATAGTTTAAACCGAA
GGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCC
TTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGT
GGAGATATCAATTCAAATCCACA
   
Sequence Name 09-K115186-0022-159-C01C-3144
GenBank Accession LN490311 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115186-0022-159-C01C-3144
CAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGAT
CAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTCGGGTAAAAATTTAAATAT
TTGTGAAATGGAGAAAAGTAAAAATTTATCATGACAGACGTCTTCATTTCCCTTAATCTT
AATCTTAACATAGTAAAAATCTCTTTCTTCAATTTATCTATATATAAGATTTTACTTTAG
TTATGTTTTAAAATAATAATATACTCTTAATTGGGTTATAAATGTGAAATCTATATATTT
TTAAAAGGGAGCAAAATCAAATCTTATGG

Last Updated on 06.12.2018 16:11