Sequences from confirmation amplicon


Confirmation sequences for line 571E02 and hit At2g17290 are shown.
Line ID 571E02
Locus At2g17290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7517717
Primers used for PCR TR06 - 8474, show primer details
   
Sequence Name 29-K027064-022-571-E02C-8409
GenBank Accession LN499142 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K027064-022-571-E02C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAAGAAAACAGAGAGACCAAAATCAAT
GGCCTTAAGAGAGAAATCATCATCCTTATTAACCAACAAGAAATTCTCAGGCTTTAAATC
TCTATGCATAACACCAAGAGAATGACACGCCTCAACAACACCGACAATGATCTTGGTCAA
CTCAGCAGCTTTCCTCTCGCTGTAATGACCTCTATGAATAATCCTATCAAACAACTCACC
ACCAGCACAAAGCTCCATCACAATGTGAACATACAAAGGATCCTCATAAGCTCCTTTAAT
AGTAACAATATTCTTGTGACCAGCTAAATGATGCATAATCTGAATCTCCCTCCTAACATC
CTCAACATCTTCTTTAGATATCAATTTCCTCTTGGATATAGACTTACAAGCATAGTCAAC
ACCTGTGGCAATATCAGTACACAAATACGTTGTCCCGAATTGTCCTTGTCCTAACTTACG
ACTCAACGTGTAAAGATCACGAATGTTAGGAGTCTTGTGACCAAGAACATAGTAAGATTG
ATTGTCAACGTTACGCCGCATAATGGGTTCTTTAACAGGAATCACAANGAGCTGGATTGG
GTGTTCTGTTTTGGAGA
   
Sequence Name 30-K027064-022-571-E02C-TR06
GenBank Accession LN499577 [GenBank]
Sequencing primer gene specific primer, TR06: 5'-AGACCAAGATTTCTCCAAACAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K027064-022-571-E02C-TR06
CACCAATCCAGCTCTTGTGATTCCTGTTAAAGAACCCATTATGCGGCGTAACGTTGACAA
TCAATCTTACTATGTTCTTGGTCACAAGACTCCTAACATTCGTGATCTTTACACGTTGAG
TCGTAAGTTAGGACAAGGACAATTCGGGACAACGTATTTGTGTACTGATATTGCCACAGG
TGTTGACTATGCTTGTAAGTCTATATCCAAGAGGAAATTGATATCTAAAGAAGATGTTGA
GGATGTTAGGAGGGAGATTCAGATTATGCATCATTTAGCTGGTCACAAGAATATTGTTAC
TATTAAAGGAGCTTATGAGGATCCTTTGTATGTTCACATTGTGATGGAGCTTTGTGCTGG
TGGTGAGTTGTTTGATAGGATTATTCATAGAGGTCATTACAGCGAGAGGAAAGCTGCTGA
GTTGACCAAGATCATTGTCGGTGTTGTTGAGGCGTGTCATTCTCTTGGTGTTATGCATAG
AGATTTAAAGCCTGAGAATTTCTTGTTGGTTAATAAGGATGATGATTTCTCTCTTAAGGC
CATTGATTTTGGTCTCTCTGTTTTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA

Last Updated on 06.12.2018 16:11