Sequences from confirmation amplicon


Confirmation sequences for line 652F05 and hit At2g18790 are shown.
Line ID 652F05
Locus At2g18790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8140311
Primers used for PCR 87yp - 8474, show primer details
   
Sequence Name 26-K121793-0022-652-F05C-87yp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 87yp: 5'-TTTATGATTCTATTCCGCATGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K121793-0022-652-F05C-87yp
CATATGTGATTCTATATTCTATCTAGTACAAATTATGAGAAGTATATACGTACGATGAGA
GTATAAAACATTTCAATATTTGTATAGAGAGGACACCACTTGGTTGACTTGACCCACGAT
AAGATATTGAAGAAACCAAACTTGTATAGTACGAATTCGAAATCGTAATTGATGATGCGA
TTCGACAAGTCCAGGGGCTCCCTCCCACGCGCAATGGGCCCAGCAACCACGTGTGGCCAC
TAGAGAGAATAAACCATTAGCCCACGTGATCTTGGGCCCAATCAATCTCTCCCTCACATT
AAACGACAAAACAAAAGCTCTTCTGGGTTAAATTGATAAATATCAAAACTTTAAAGGTAA
TTTGCTAAAATCGCCACACAAAAAAAGTCGCAGAAAATATATGAGGAAACAAAAAGCGAA
GACGACAAAAAAAAAAAAACCCCGAATTTTTTTTGGTACCCCCCCCCAACGGAAAGGGCC
CAATTTTGGCTCCCCCTCCTCAATTTTTTTTTAGGGGTTCCCCCCTTTTTCCCAAATCCA
ATTTCCCCCTTTTTTTTTCTCCCCAAGTTCAAAATTTTTGGAAATTTTCCTTCCCCAAAA
TTTCTCCCCAAAAAACAGGGGAAGGAGATTCCCCCCAAATCCTTCCTTGGTCCAGGGGAA
TTTTGAAAAATTTTCTAAATTTAAAAACCAACGGGCTGGTTTCCGGAATCCGGGGTAAGG
GCGGGGGCCCTGGCGGGGGCCCGGGCAAAAAAAAAAAACCCCCCTCAATTTCCCCCCCTT
AAAACCCAAAAG
   
Sequence Name 10-K121792-0022-652-F05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K121792-0022-652-F05C-8409
TTCATTGAATATATCCTGATTCGCCGGATTGTTCGAAAACGGCGTGGAGTCTTGCGTCGA
CGGTGTACTGTTGAATTGCTTTGCTCATTGATTCAGTGTTGCTTCTTGGTCTGAGAGATT
TCGTTCCCGACGATTGAGCTTGTTCTCCTCCTCTTCGGTTATTAGGAGTGTGACTTGACG
ACGGTTCTTCTTCTCCGCCACGGCCACCGCCACGGCCACCGCCACTACCCCCGACTCCGG
AAACCATGCCGTTTGATTTTGAATTTGAGAAATTTCTCAGAATTACCTTGAGACAAGGAA
GGATTTAGGGTGAATCATCATCCACTAGTTATCGGAGACGAATTCTGGTAGAGCTAAATT
CTCAAGAATTTTGAACTTGAGGAAGAAGAAAATGGGGAGAATTGAGATCGGAGATAAGTG
GAGAAACCAATAAAATAAATTGAAGAAGACGAAGCAAAATGTGTGCCTCTCAGATAGAGA
GAGATAACAAAAAAAAATCAGAGTTTTTTTTTTTTTGGCCCCCTCCCTTTTTTTTTCCCC
CAAATTTTTCCGGCAATTTTTTTGGGGGGGGATTTTAACAAATTCCCTTTAAAGTTTTGA
ATTTTTCCCATTTACCCCCAAAAAATTTTTTTTTTTCCCTTTAAGGGGGGGGAAAAATTT
TTTGGGCCCCAAAACCCGGGGGGCAAGGGTTTTTTTCCCTCTGGGGCCCCCCGTGGTTTG
GGGGCCCTTTGGGGGGGGGAGGGGACCCCCGGAATTTGCAAAACCCCACCCCAATTAAAA
TTTCCAAT

Last Updated on 06.12.2018 16:11