Sequences from confirmation amplicon


Confirmation sequences for line 614F01 and hit At2g19760 are shown.
Line ID 614F01
Locus At2g19760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8517512
Primers used for PCR BF49 - 8474, show primer details
   
Sequence Name 23-K029004-022-614-F01C-8409
GenBank Accession LN496429 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K029004-022-614-F01C-8409
CATTTACAATTGAATATATCCTGATTTGATCAATAGCCTAAAATGTCGATTTGATTTTGC
CAAAATCTGATGATGTGTTATTGATAATGTGTGTTTAGTTGAAGCCTCAAGAAATCGATG
GAATCAAGAAGGACTTTGAGGAGCCCGGGTTTCTTGCCCCAACCGGACTATTTCTCGGTG
GCGAAAAATACATGGTTATCCAAGGTGAACAAGGAGCTGTGATCCGAGGGAAGAAGGTAA
CTTTCTTTACTTCATACATCAGAAAGCTGCATGTAGATTTTGATAGAGAATAGAATCGGA
ATTCATGTAACAATCTGTGAATCTTCAGGGACCTGGAGGTGTCACTATCAAGAAGACAAA
CCAAGCTTTGGTCTTTGGCTTCTACGATGAACCAATGACTGGAGGTCAATGCAACTTGGT
TGTCGAAAGGCTCGGGGATTACCTTATCGAGTCTGAACTCTAAAACCAAGGTTTCATTTC
AGGTTCTTCTTAACTAAAGAGTGTCAATGCACTTTTTATTGTGATTGATTGTAATGCTTT
CAAACACAAATCATTTGTTACTTTAGAACCAATTGTGATTGATTGGTCTCCTTCGTTACC
GAGTTTGA
   
Sequence Name 24-K029004-022-614-F01C-BF49
GenBank Accession LN496872 [GenBank]
Sequencing primer gene specific primer, BF49: 5'-GAACACACAAACTCAAACTCGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K029004-022-614-F01C-BF49
ACAAATGATTTGTGTTTGAAAGCATTACAATCAATCACAATAAAAAGTGCATTGACACTC
TTTAGTTAAGAAGAACCTGAAATGAAACCTTGGTTTTAGAGTTCAGACTCGATAAGGTAA
TCCCCGAGCCTTTCGACAACCAAGTTGCATTGACCTCCAGTCATTGGTTCATCGTAGAAG
CCAAAGACCAAAGCTTGGTTTGTCTTCTTGATAGTGACACCTCCAGGTCCCTGAAGATTC
ACAGATTGTTACATGAATTCCGATTCTATTCTCTATCAAAATCTACATGCAGCTTTCTGA
TGTATGAAGTAAAGAAAGTTACCTTCTTCCCTCGGATCACAGCTCCTTGTTCACCTTGGA
TAACCATGTATTTTTCGCCACCGAGAAATAGTCCGGTTGGGGCAAGAAACCCGGGCTCCT
CAAAGTCCTTCTTGATTCCATCGATTTCTTGAGGCTTCAACTAAACACACATTATCAATA
ACACATCATCAGATTTTGGCAAAATCAAATCAACATTTTAGGCTATTGATCAAATCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTA

Last Updated on 06.12.2018 16:11