Sequences from confirmation amplicon


Confirmation sequences for line 493G05 and hit At2g19930 are shown.
Line ID 493G05
Locus At2g19930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8612329
Primers used for PCR HN03 - 8474, show primer details
   
Sequence Name 14-K035349-022-493-G05C-HN03
GenBank Accession LN492414 [GenBank]
Sequencing primer gene specific primer, HN03: 5'-CTCCGACCTTTTTATTTCCTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K035349-022-493-G05C-HN3
ATCAATCCATCACTAACAGTAGAAACTGTTTCTCTACTGGGTATTTTTTTTTGGCAACCC
TTAAATTCTGAAGTAAGAGAGAAGAAGAAGAAAGTGTGAGATTCCGACGAACGGGAAGAA
AAATGATAAATTTTCGCAGCATGAATCAAGCTTCTTCGAGGATAAGGATCGCTCTTTCGG
GTCGGATTGAAACAGCTTTAGAGAATATCTACAGAAAACACAATCTTACTCCGATTAATG
ATGAGACGAGACAATATGAAAAAGAAAGAGTAATTACCAATATGGCTTCAATTGGTAAAT
AAAAAATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTCACTCCCAAATGG
   
Sequence Name 06-K035349-022-493-G05C-8409
GenBank Accession LN488711 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K035349-022-493-G05C-8409
CGGACTGAGCCATTTACATTGAAGCCATTTTTTATTTACCAATTGAAGCCATATTGGTAA
TTACTCTTTCTTTTTCATATTGTCTCGTCTCATCATTAATCGGAGTAAGATTGTGTTTTC
TGTAGATATTCTCTAAAGCTGTTTCAATCCGACCCGAAAGAGCGATCCTTATCCTCGAAG
AAGCTTGATTCATGCTGCGAAAATTTATCATTTTTCTTCCCGTTCGTCGGAATCTCACAC
TTTCTTCTTCTTCTCTCTTACTTCAGAATTTAAGGGTTGCCAAAAAAAAATACCCAGTAG
AGAAACAGTTTCTACTGTTAGTGATTGGATTTGATTTGACGATGACACAAGAAGAAACAG
AGAGGAAATAAAAAGGTCGGAGAAGCTATCGCTTCTCTAA

Last Updated on 06.12.2018 16:11