Sequences from confirmation amplicon


Confirmation sequences for line 004D10 and hit At2g20490 are shown.
Line ID 004D10
Locus At2g20490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8832574
Primers used for PCR MG43 - 8474, show primer details
   
Sequence Name 56-K032523-022-004-D10C-8409
GenBank Accession LN509009 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K032523-022-004-D10C-8409
GATTCGTGAAGTAACAAAACCTTTAAACCAAATGTTTTGTGTTGTTTGCAGCCCGGTTCT
CCCCTGACGATAAATACTCAAAGCAGAGAGTATTGTTGAAGAAGCGATTTGGTCTCTTAC
CGACCCAAAACGCACCTCTCCAGTACTGATGAAGAAGCAAACCACTTGCCTGTGTTGGTT
TTTGATGTTCCTTTTTAATTTGCTATGTTGTCTCTTAATGAAATGTTGACTTGAACCGTC
TTTGTAATGTCTTCAAATAGTGTGAGTTAAGAGCAATTTTTACCTATCGTCGTCAAGCCT
GCCACTTAAAGATTAATTTACATTAAGAATTTGTGAGTGCCCATTTATGTTGCTTTGTTT
CTTGTCAACTAGAAACTTCATTAATGGCTCAAGTAAGAAACCATAAACAGAGTATCATAT
CAAAGGTAAGTAAACCAGAGAGAAGATCATCAACAAAATAAAAATTTACACCTGTAAAAC
CTCAAATTCTCGAATTGAAACAAATTAAAGTTATGTTTGGGCCCTTTTGTTCCAGGCCCA
TGAAAATTGTCCGTGCCGACAAAGTCAGTTTTTTTTGGTTCTTCGAGAAAGAAAAGAAAA
ACAAAATGAAGAAAAGAACAAATCCGGAATCGTCCACCTCTGAAGCAAAGAAACGCCGGC
GAGAGGAAGATGACGATAAAGTTGGTTGTAGTCACT
   
Sequence Name 56-K032523-022-004-D10C-MG43
GenBank Accession LN509010 [GenBank]
Sequencing primer gene specific primer, MG43: 5'-TTCAATTGCAGAGTGACTACAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K032523-022-004-D10C-MG43
TTGCTTCGAGGTGGACGATTCCGGATTTGTTCTTTTCTTCATTTTGTTTTTCTTTTCTTT
CTCGAAGAACCAAAAAAAACTGACTTTGTCGGCACGGACAATTTTCATGGGCCTGGAACA
AAAGGGCCCAAACATAACTTTAATTTGTTTCAATTCGAGAATTTGAGGTTTTACAGGTGT
AAATTTTTATTTTGTTGATGATCTTCTCTCTGGTTTACTTACCTTTGATATGATACTCTG
TTTATGGTTTCTTACTTGAGCCATTAATGAAGTTTCTAGTTGACAAGAAACAAAGCAACA
TAAATGGGCACTCACAAATTCTTAATGTAAATTAATCTTTAAGTGGCAGGCTTGACGACG
ATAGGTAAAAATTGCTCTTAACTCACACTATTTGAAGACATTACAAAGACGGTTCAAGTC
AACATTTCATTAAGAGACAACATAGCAAATTAAAAAGGAACATCAAAAACCAACACAGGC
AAGTGGTTTGCTTCTTCATCAGTACTGGAGAGGTGCGTTTTGGGTCGGTAAGAGACCAAA
TCGCTTCTTCAACAATACTCTCTGCTTTGAGTATTTATCGTCAGGGGAGAACCGGGCTGC
AAACAACACAAAACATTTGGTTTAAAGGTTTTGTTACTTTCACGAATCATAACTAAAACA
ACTCAAAAGGTCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11