Sequences from confirmation amplicon


Confirmation sequences for line 321D12 and hit At2g20750 are shown.
Line ID 321D12
Locus At2g20750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8941658
Primers used for PCR 25ns - 8474, show primer details
   
Sequence Name 74-K114626-0022-321-D12C-25ns
GenBank Accession LN513124 [GenBank]
Sequencing primer gene specific primer, 25ns: 5'-CCAACAATTCTTTCTCACTTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K114626-0022-321-D12C-25ns
TTTTTTTGTTGGCAGTCTCTTCGGTCAAAATGCAGCTCTTTCCGGTCATCCTACCGACAC
TATGCGTCTTCCTCCACCTTCTAATAAGTGGCTCTGGCTCTACTCCACCGTTGACTCACT
CCAATCAACAAGTGGCAGCCACTCGTTGGCTTCCCGCCACCGCAACCTGGTACGGAAGTG
CCGAGGGAGACGGCAGCAGTGGTAAACATCTCTTACTTTTTTACTCACAATTTGTTTGGT
TTTTACTGTAACAGACCAAAAGTTTACTGCGAAATCAATAATAATGCAAGGGCTTAATTA
TGAATGAAACCGCAGGAGGAGCTTGTGGTTACGGATCGTTGGTGGACGTGAAGCCGTTTA
AGGCTAGAGTCGGAGCGGTGAGTCCGATTCTGTTCAAAGGTGGTGAAGGCTGCGGTGCAT
GCTACAAGGTCAGGTGTCTCGACAAGACCATTTGCTCTAAGAGAGCAGTCACCATTATTG
CCACCGACCAGTCACCGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATTCC
   
Sequence Name 74-K114625-0022-321-D12C-8409
GenBank Accession LN513123 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K114625-0022-321-D12C-8409
GCCTTACATTGAATAATCCTGACGGTGACTGGTCGGTGGCAATAATGGTGACTGCTCTCT
TAGAGCAAATGGTCTTGTCGAGACACCTGACCTTGTAGCATGCACCGCAGCCTTCACCAC
CTTTGAACAGAATCGGACTCACCGCTCCGACTCTAGCCTTAAACGGCTTCACGTCCACCA
ACGATCCGTAACCACAAGCTCCTCCTGCGGTTTCATTCATAATTAAGCCCTTGCATTATT
ATTGATTTCGCAGTAAACTTTTGGTCTGTTACAGTAAAAACCAAACAAATTGTGAGTAAA
AAAGTAAGAGATGTTTACCACTGCTGCCGTCTCCCTCGGCACTTCCGTACCAGGTTGCGG
TGGCGGGAAGCCAACGAGTGGCTGCCACTTGTTGATTGGAGTGAGTCAACGGTGGAGTAG
AGCCAGAGCCACTTATTAGAAGGTGGAGGAAGACGCATAGTGTCGGTAGGATGACCGGAA
AGAGCTGCATTTTGACCGAAGAGACTAGCCAACAAAAAAACTGACACTAACCGAGAAAAA
AAGTGGAGAAGTGAGAAGAAATTTGTTGG

Last Updated on 06.12.2018 16:11