Sequences from confirmation amplicon


Confirmation sequences for line 436D04 and hit At2g22630 are shown.
Line ID 436D04
Locus At2g22630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9619350
Primers used for PCR SZ03 - 8474, show primer details
   
Sequence Name 24-K020243-022-436-D04C-8409
GenBank Accession LN496814 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K020243-022-436-D04C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGATATAATACTTCATA
ATAAATTGTGCAGTCATTTATGGTTTAAAATACATAGTCATCTTATCGTTCTTGGGTAGT
TTCATATATACAAAATATTGAGATTTATATGCTTATATACAAGTAGATGGGTAATTTTTT
TATCAGTTTACATATATCATCTCCAAATGTATGATATATCCTTAAAAATGCACAATTGAT
TGTAGTGATATCTTATACACTCCCATTTATCACTCTTTCAGTTTTCCAACGTTATACTTG
TTTTTAGCTTTAATTACTATCTTCTCTACTCAAATGTTTCTAAATTTGGCAAAGACACAC
TGTCAGCCACATACACAATTTACAACCAATGACACACATGTCCACACACTGCCATGAGCC
TACTTCAAATTAGTGTGTGTTTTCATCTTTACACTAGTCATCGTCTTTAATTAGTTTTCT
TTTGCTTTGGCATCCTAAGCTCC
   
Sequence Name 28-K020243-022-436-D04C-SZ03
GenBank Accession LN498653 [GenBank]
Sequencing primer gene specific primer, SZ03: 5'-TAGTTTGAAAGGAGCTTAGGATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K020243-022-436-D04C-SZ03
CAAAGCAAAAGAAAACTAATTAAAGACGATGACTAGTGTAAAGATGAAAACACACACTAA
TTTGAAGTAGGCTCATGGCAGTGTGTGGACATGTGTGTCATTGGTTGTAAATTGTGTATG
TGGCTGACAGTGTGTCTTTGCCAAATTTAGAAACATTTGAGTAGAGAAGATAGTAATTAA
AGCTAAAAACAAGTATAACGTTGGAAAACTGAAAGAGTGATAAATGGGAGTGTATAAGAT
ATCACTACAATCAATTGTGCATTTTTAAGGATATATCATACATTTGGAGATGATATATGT
AAACTGATAAAAAAATTACCCATCTACTTGTATATAAGCATATAAATCTCAATATTTTGT
ATATATGAAACTACCCAAGAACGATAAGATGACTATGTATTTTAAACCATAAATGACTGC
ACAATTTATTATGAAGTATTAT

Last Updated on 06.12.2018 16:11