Sequences from confirmation amplicon


Confirmation sequences for line 031A11 and hit At2g23070 are shown.
Line ID 031A11
Locus At2g23070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9826630
Primers used for PCR 06jx - 8474, show primer details
   
Sequence Name 61-K113261-0022-031-A11C-06jx
GenBank Accession LN510570 [GenBank]
Sequencing primer gene specific primer, 06jx: 5'-CACATAGACCAGCAATTTTCAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K113261-0022-031-A11C-06jx
ACAATCAGATACCAATTCTGCATTACTTACGAGATTATATCATCAATCAAAAATATGAAA
CGAAACCCTAAATCTACATTCCACATTGATTCCAGAACAACCAATCCCAAACCAATCTAT
AACCAAAGGCAAACATCTAGAAATCGACGTAATTAGGTTTAATTAGAGAAGAATCTGAAG
ATATACCCCCATTGAACAGCAAGGGACTCGTAATCCCAATAGTCCTTAGGTCTAACAACG
TTGACATCAGCGTAAACCCTAGCTTTCGACGGCGCACCGGCACGCCGGATAGATTTACCG
ATCTTCTGAGCCAGCGTTTCTGATTACGACAATGGCTTCATATATTGACGCATATGACGT
TCTCAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATT
   
Sequence Name 42-K113260-0022-031-A11C-8409
GenBank Accession LN504529 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K113260-0022-031-A11C-8409
TTGAGACGTCTATGCGTCAATATATGAAGCCATTGTCGTAATCAGAAACGCTGGCTCAGA
AGATCGGTAAATCTATCCGGCGTGCCGGTGCGCCGTCGAAAGCTAGGGTTTACGCTGATG
TCAACGTTGTTAGACCTAAGGACTATTGGGATTACGAGTCCCTTGCTGTTCAATGGGGGT
ATATCTTCAGATTCTTCTCTAATTAAACCTAATTACGTCGATTTCTAGATGTTTGCCTTT
GGTTATAGATTGGTTTGGGATTGGTTGTTCTGGAATCAATGTGGAATGTAGATTTAGGGT
TTCGTTTCATATTTTTGATTGATGATATAATCTCGTAAGTAATGCAGAATTGGTATCTGA
TTTGTTTGTATTGGTATCTGTTTTGTTTGTATATAATCTCTTGAAAATTGCTGGTCTATG
TG

Last Updated on 06.12.2018 16:11