Sequences from confirmation amplicon


Confirmation sequences for line 377E12 and hit At2g23070 are shown.
Line ID 377E12
Locus At2g23070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9826892
Primers used for PCR CD21 - 8474, show primer details
   
Sequence Name 11-K115282-0022-377-E12C-CD21
GenBank Accession LN491247 [GenBank]
Sequencing primer gene specific primer, CD21: 5'-TTCCACCGAAAATTTATCAAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K115282-0022-377-E12C-CD21
GAAATTCTAAAGCTAAAGTTTACCCCCATTGAACACGAGAGACTCGTAGTCCCAATACTC
TTTAGGACGAACAACGTTAACATCTGTATAAACCCTAGCTTTCGACATCAATTTCGATCT
GAAAAAATGGAGAAATTTGCGATCGCAATCTTCTCTTTGATCGTCAGGTTTGTTTCAAGT
GGACGATGATGATGACAACAGAAGAAGTGCGTTGTGCAGGATTAGAAAAAGGTTGAAGAA
GAATCTCTGATACCCAAAAACCACCTAAACCAAATCGAACCGATTCAACCACACTCGAAC
CGAAACAAACCCAATCGGATCGATTTCAATCACACTATAACCGGACCGAATTGTTTAAAC
GACATCGTTTCGAAGAGAAGCCAAAGAAAAAGAAAGGAGAGAAATAAAGCAAATTCGTTA
CCTTCACTTTTCATTTTCTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 02-K115281-0022-377-E12C-8409
GenBank Accession LN487160 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115281-0022-377-E12C-8409
CATTTACATTGAATATATTCAGAAAATGAAAAGTGAAGGTAACGAATTTGCTTTATTTCT
CTCCTTTCTTTTTCTTTGGCTTCTCTTCGAAACGATGTCGTTTAAACAATTCGGTCCGGT
TATAGTGTGATTGAAATCGATCCGATTGGGTTTGTTTCGGTTCGAGTGTGGTTGAATCGG
TTCGATTTGGTTTAGGTGGTTTTTGGGTATCAGAGATTCTTCTTCAACCTTTTTCTAATC
CTGCACAACGCACTTCTTCTGTTGTCATCATCATCGTCCACTTGAAACAAACCTGACGAT
CAAAGAGAAGATTGCGATCGCAAATTTCTCCATTTTTTCAGATCGAAATTGATGTCGAAA
GCTAGGGTTTATACAGATGTTAACGTTGTTCGTCCTAAAGAGTATTGGGACTACGAGTCT
CTCGTTGTTCAATGGGGGTAAAACTTCTAGCTTTAGAATTTTTCTTTGATGAATTTACGA
TTTTTGACTTCTTTTTGATAAATTTCCGGTGGA

Last Updated on 06.12.2018 16:11