Sequences from confirmation amplicon


Confirmation sequences for line 162C10 and hit At2g24070 are shown.
Line ID 162C10
Locus At2g24070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10231946
Primers used for PCR 19xy - 8474, show primer details
   
Sequence Name 28-K119596-0022-162-C10C-19xy
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 19xy: 5'-CATGCAATATCAGACCTAAGGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K119596-0022-162-C10C-19xy
TTGTCTCACAGTTGAAGCTGAAATCAGTTAAGATCCATCTTAAATGATCAGGTATGTCTT
CACTCGCACGCATTTGTGTATTTGTGTTCATCGAAGTATCATCCTCATGATCTAAATGTA
TTTATTTGCGCAGATGGTTTGCCTTGAGGATTGGGCAATGGTTGAGAGAGAACACATTAG
TTCTTTAGCTGGAGCCATTGGAGACTTAGAAGCAAATACACTCCGGCTTCCACTAGCCGG
AGGAACAAAGGTACGGTTTACGTTTTCCGAGAAAACTAGAAAACAAGTCGAAGAGCAAGA
ACCGAGTTCTGATTTTGTAAAAGCTCTTGTGATCACAGGCAGACCTAGGATCTCTGAAGT
TAGCTATGTCCTCAGCTCTTGATGTAATGCAGAGTATGGGATCATCCATCTGGTCTTTAC
ATTCGCAGATGGAGGAGATGAACAAACTTGTTTCGGATCTCGCTGTTATAGCTAAAACAG
AGAATTTTTTGCTTGACAAATGTGAAAATCTCTTGGCATCAACTGCAGTCATGGAGGTAA
CAAATCAAAAATACAAATCCAAGAATCTAATGACTAATATAGTTCAACACGTTAAACCGA
GTTTTTCGTCGTTAAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 04-K119595-0022-162-C10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K119595-0022-162-C10C-8409
TCATTGAATATATCCTGTTAACGACGAAAAACTCGGTTTAACGTGTTGAACTATATTAGT
CATTAGATTCTTGGATTTGTATTTTTGATTTGTTACCTCCATGACTGCAGTTGATGCCAA
GAGATTTTCACATTTGTCAAGCAAAAAATTCTCTGTTTTAGCTATAACAGCGAGATCCGA
AACAAGTTTGTTCATCTCCTCCATCTGCGAATGTAAAGACCAGATGGATGATCCCATACT
CTGCATTACATCAAGAGCTGAGGACATAGCTAACTTCAGAGATCCTAGGTCTGCCTGTGA
TCACAAGAGCTTTTACAAAATCAGAACTCGGTTCTTGCTCTTCGACTTGTTTTCTAGTTT
TCTCGGAAAACGTAAACCGTACCTTTGTTCCTCCGGCTAGTGGAAGCCGGAGTGTATTTG
CTTCTAAGTCTCCAATGGCTCCAGCTAAAGAACTAATGTGTTCTCTCTCAACCATTGCCC
AATCCTCAAGGCAAACCATCTGCGCAAATAAATACATTTAGATCATGAGGATGATACTTC
GATGAACACAAATACACAAATGCGTGCGAGTGAAGACATACCTGATCATTTAAGATGGAT
CTTAACTTGATTTCTAGCTTCAACTGTTGGAGACAAATTCTTTGAGTTGTCACTAGATCC
CTTAGGT

Last Updated on 06.12.2018 16:11