Sequences from confirmation amplicon


Confirmation sequences for line 458B09 and hit At2g26670 are shown.
Line ID 458B09
Locus At2g26670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11341762
Primers used for PCR NJ16 - 8474, show primer details
   
Sequence Name 92-K111634-0022-458-B09C-NJ16
GenBank Accession LN515201 [GenBank]
Sequencing primer gene specific primer, NJ16: 5'-CCACAAGAAACCTCAAGTAACCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K111634-0022-458-B09C-NJ16
TCATTTGCACGGACGTTCCTCATAGATTTAGTCTCTTTCTCACCTTCCTTAGCTTGATCT
TTAGTATGATGTCTCATAGCCACAAACCTCATCTCCTCCACAAAACCCTTTGATTCTCCA
GGATACCTCTTCTTCTGCTTCTCTGCCGCAGTAGTAGCCGCAACCACCACTAAAGACGGC
GACTTGTTCATGGTCATACTCAGAATCTGAATCCTAGGTCGAAGGAAAAGTGGGTTCGGT
GAGGAAGAAGAAAATTGGAATCTTGAGAGTTGGGGATTCTTGAATATGGATAAGGATGAA
GAAATCGGAGCTAAATACGCCATGGTTTGATCGGAATAGAAAAATGGTTACAGAAGAAGT
CACGCAATGTAGTTTTAATTGAAGGAATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCACGGNTTATT
   
Sequence Name 84-K111633-0022-458-B09C-8409
GenBank Accession LN514448 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K111633-0022-458-B09C-8409
TCATTGAATATATTCCTTCATTAAAACTACATTGCGTGACTTCTTCTGTAACCATTTTTC
TATTCCGATCAAACCATGGCGTATTTAGCTCCGATTTCTTCATCCTTATCCATATTCAAG
AATCCCCAACTCTCAAGATTCCAATTTTCTTCTTCCTCACCGAACCCACTTTTCCTTCGA
CCTAGGATTCAGATTCTGAGTATGACCATGAACAAGTCGCCGTCTTTAGTGGTGGTTGCG
GCTACTACTGCGGCAGAGAAGCAGAAGAAGAGGTATCCTGGAGAATCAAAGGGTTTTGTG
GAGGAGATGAGGTTTGTGGCTATGAGACTTCATACTAAAGATCAAGCTAAGGAAGGTGAG
AAAGAGACTAAATCTATTGAGGAACGTCCTGTTGCTAAATGGGAACCTACTGTTGAAGGT
TACTTGAGGTTTCTTGTGG

Last Updated on 06.12.2018 16:11