Sequences from confirmation amplicon


Confirmation sequences for line 320A06 and hit At2g26770 are shown.
Line ID 320A06
Locus At2g26770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11404008
Primers used for PCR 12bd - 8474, show primer details
   
Sequence Name 55-K118893-0022-320-A06C-12bd
GenBank Accession LN508954 [GenBank]
Sequencing primer gene specific primer, 12bd: 5'-AGTATGAAACTGATGTGTTGCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K118893-0022-320-A06C-12bd
GTCAAGCCCTTGAGAACGGCTCTTACCTAAGTTGTGATTTTTAGTATCCGTAAGAGCTTA
TTTGTTTTGTAGTTTATATAGGTTTTAGGCATTTGTTTGAATTGTACTTGTATTGTGTGT
AAAAATATCGAGCAGTGAAGTAAGAGGTGATGGTGAATCTATATGTAACGGGTGGCTTGT
GTTTATTCATTTGTTTTGATCTTACTTGCCCTTGAGGTAATATAACGGATTGCTTGTTTT
GGACTGGAAATATTGAATTGAGGTAATATTGTATGACATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 15-K118892-0022-320-A06C-8409
GenBank Accession LN493200 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118892-0022-320-A06C-8409
CATTTACATTGAATATGTCATACAATATTACCTCAATTCAATATTTCCAGTCCAAAACAA
GCAATCCGTTATATTACCTCAAGGGCAAGTAAGATCAAAACAAATGAATAAACACAAGCC
ACCCGTTACATATAGATTCACCATCACCTCTTACTTCACTGCTCGATATTTTTACACACA
ATACAAGTACAATTCAAACAAATGCCTAAAACCTATATAAACTACAAAACAAATAAGCTC
TTACGGATACTAAAAATCACAACTTAGGTAAGAGCCGTTCTTCAAGGGCTTTGACCCGTC
TCTGTTCTGTCTTCTGGACCAGCAAGTGTCACCTGCAACACATCAATTTCATAC

Last Updated on 06.12.2018 16:11