Sequences from confirmation amplicon


Confirmation sequences for line 304D01 and hit At2g26820 are shown.
Line ID 304D01
Locus At2g26820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11440999
Primers used for PCR ZZ33 - 8474, show primer details
   
Sequence Name 34-K020358-022-304-D01C-8409
GenBank Accession LN501238 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K020358-022-304-D01C-8409
TAGATTTCCTGGGGAAATGAGTATTTCAATAATAAAAATCTATGTACTATAGCAACTTAA
AAAGGATAGTTTGTTCGTGATATCGTAATACATTAGATTGTTTGTTAAAGAATATGAATT
TACCGCTTGTGCAATCAACTGTTTGTTTTTCGCCTCCTCCATTTGTAACTTCTCCTTTTC
CATTAGTAAATTCTGTTGCATCTCTGCTAGTTCTGCTTCAGCACGGTTTTTAGATTCAAT
CACCCTTTCTTCTTCCCTTAGCCTTTCATCATTTTCTTCCTGGAAAAATCAAGATTTTCA
ATAACTACATATGTATGTACTATAGCAATTTCTGTTAAGATAGTTTATTTGTGACAGCAT
ATGCTAGACTAGATTGCTTTTATTTGATGTGGTTAACATG
   
Sequence Name 38-K020358-022-304-D01C-ZZ33
GenBank Accession LN502832 [GenBank]
Sequencing primer gene specific primer, ZZ33: 5'-ACCATGTCAACCACATCAAATAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K020358-022-304-D01C-ZZ33
TCTAGTCTAGCATATGCTGTCACAAATAAACTATCTTAACAGAAATTGCTATAGTACATA
CATATGTAGTTATTGAAAATCTTGATTTTTCCAGGAAGAAAATGATGAAAGGCTAAGGGA
AGAAGAAAGGGTGATTGAATCTAAAAACCGTGCTGAAGCAGAACTAGCAGAGATGCAACA
GAATTTACTAATGGAAAAGGAGAAGTTACAAATGGAGGAGGCGAAAAACAAACAGTTGAT
TGCACAAGCGGTAAATTCATATTCTTTAACAAACAATCTAATGTATTACGATATCACGAA
CAAACTATCCTTTTTAAGTTGCTATAGTACATAGATTTTTATTATTGAAATACTCATTTC
CCCAGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11