Sequences from confirmation amplicon


Confirmation sequences for line 764B10 and hit At2g27460 are shown.
Line ID 764B10
Locus At2g27460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11741095
Primers used for PCR NA41 - 8474, show primer details
   
Sequence Name 82-K101673-0022-764-B10C-8409
GenBank Accession LN514168 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K101673-0022-764-B10C-8409
TTCGGAATGAGCCATTTACATTGAATATATCCTGAACGATCCATCCACTAGAAAAACAAA
CAATTAATCAGAAATTAGAACCAATTCCAACAAAAAAAACAAAAGAATCAGTATAGAGAT
GACGTAAGACATACGAGGCATCTCAAGATCAATGAAGGAGGAAGTCATTTCAGGTATAGA
GTGAGGATTAGAGTAACGAGCAATAGCATCAGAAGGAAGTCCATTGAGAGTTCCACAAAG
CGAGCAATTCCAAGCCCATTGATCAAGCTCACAATAGGTATTGAAGTAAGCGTAGCAGTT
TTCGCAACGAGGAAGAAGATGACCGTTAGATCCGCACGCTGGTCCGATTCCGTTCTCGTC
TTTGGCCGCGAATGGTGTTACTGTCAGTCCCCAAGGAAGTCCTGACGCCTCTTGAGCGTC
TGAATCGATCGGAAAACGCGACACCGTTGCTCTCACTGCCATTGTTTCAGATCAGATCCT
TCCAAATGTGAAAATTTTGATTCTGGTTTTAAGGTTTTCGGAGATTTGGTGAAATTGGAT
GAGCACGGTGGAAATTAGGCGCACGTTAGCTTTTCCGAAAAAAATTC
   
Sequence Name 82-K101680-0022-764-B10C-NA41
GenBank Accession LN514169 [GenBank]
Sequencing primer gene specific primer, NA41: 5'-CGAATTTTACTGGAAAAGCTAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K101680-0022-764-B10C-NA41
ATTTCCCAATCTCCGAAAACCTTAAAACCAGAATCAAAATTTTCACATTTGGAAGGATCT
GATCTGAAACAATGGCAGTGAGAGCAACGGTGTCGCGTTTTCCGATCGATTCAGACGCTC
AAGAGGCGTCAGGACTTCCTTGGGGACTGACAGTAACACCATTCGCGGCCAAAGACGAGA
ACGGAATCGGACCAGCGTGCGGATCTAACGGTCATCTTCTTCCTCGTTGCGAAAACTGCT
ACGCTTACTTCAATACCTATTGTGAGCTTGATCAATGGGCTTGGAATTGCTCGCTTTGTG
GAACTCTCAATGGACTTCCTTCTGATGCTATTGCTCGTTACTCTAATCCTCACTCTATAC
CTGAAATGACTTCCTCCTTCATTGATCTTGAGATGCCTCGTATGTCTTACGTCATCTCTA
TACTGATTCTTTTGTTTTTTTTGTTGGAATTGGTTCTAATTTCTGATTAATTGTTTGTTT
TTCTAGTGGATGGATCGTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11