Sequences from confirmation amplicon


Confirmation sequences for line 379G06 and hit At2g27840 are shown.
Line ID 379G06
Locus At2g27840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11862694
Primers used for PCR HV13 - 8474, show primer details
   
Sequence Name 13-K031867-022-379-G06C-8409
GenBank Accession LN491921 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K031867-022-379-G06C-8409
CATTTACATTGAATATATCCCCGGATTTACACAAAGAGAGAAACTTGCAGGAATTACAAA
ACTGATACAGACCTGAGAGGCATGGACCATGAATCCATCTTTTTGTATCACCTTAAATGG
CTTCCCTGGCTTAATCTCGATACCTAAAATTGAACATTATTCATCACACATTTGAGTACA
AATTCATACAAAATCTCACAAAGATAGATGCTTTGACACCTAAACTCACAAAGATATAGA
TACTTTGACACCTAAACTCACAAAGATAGATGCTTTGAAACATAAACTCTTAAAGATAGA
TACTTTGAAACATAACCTCACAAAGATAGATGCATTGAAACCTAAACACTAAGAATGCTA
ATATTCCCAAACAATTGTATAGTTTTGAACACAAGAAAATGCGTATTGAGTAATAGAGAG
AATTCAATACAGAAGAAAGGCAAGCTTTTGTATAAAGACCAGATTTTTAATCACAGAACG
AACTTAGGAATACCAAAATGATTAACTTTAATGGCGAAAACAGTCAATAGGAGTCATTGA
GGTATTCAGTAGAACTTAATAAAGATATGATTTTGAGAGAGACATACCCCAAAACTCCAT
AGCTAGTGAAAGAGGAAGATGTGGCTGCTTGAGAAGAAGAATTTAGGGTTTTAGAGTTTG
TTTCAAACAAACAAAAATAATGGAGCGGCTT
   
Sequence Name 14-K031867-022-379-G06C-HV13
GenBank Accession LN492393 [GenBank]
Sequencing primer gene specific primer, HV13: 5'-AACCTTATAAATAAGCCGCTCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K031867-022-379-G06C-HV13
ACCCTAAATTCTTCTTCTCAGCAGCCACATCTTCCTCTTTCACTAGCTATGGAGTTTTGG
GGTATGTCTCTCTCAAAATCATATCTTTATTAAGTTCTACTGAATACCTCAATGACTCCT
ATTGACTGTTTTCGCCATTAAAGTTAATCATTTTGGTATTCCTAAGTTCGTTCTGTGATT
AAAAATCTGGTCTTTATACAAAAGCTTGCCTTTCTTCTGTATTGAATTCTCTCTATTACT
CAATACGCATTTTCTTGTGTTCAAAACTATACAATTGTTTGGGAATATTAGCATTCTTAG
TGTTTAGGTTTCAATGCATCTATCTTTGTGAGGTTATGTTTCAAAGTATCTATCTTTAAG
AGTTTATGTTTCAAAGCATCTATCTTTGTGAGTTTAGGTGTCAAAGTATCTATATCTTTG
TGAGTTTAGGTGTCAAAGCATCTATCTTTGTGAGATTTTGTATGAATTTGTACTCAAATG
TGTGATGAATAATGTTCAATTTTAGGTATCGAGATTAAGCCAGGGAAGCCATTTAAGGTG
ATACAAAAAGATGGATTCATGGTCCATGCCTCTCAGGTCTGTATCAGTTTTGTAATTCCT
GCAAGTTTCTCTCTTTGTGTAAATCCGGGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTA
Primers used for PCR FS10 - 8474, show primer details
   
Sequence Name 44-K118546w-0022-379-G06C-FS10
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, FS10: 5'-AATCTCAAACTCGTGCCCTAAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K118546w-0022-379-G06C-FS10
TTTCCAATCATAAACCCATTCTCATCATCACCAATCTTCACATAAACGGCAAAAGTCTCA
TCTTTTTTAACCTTCTCAACGTCACCAAGGGTAACCTAAGAGATGTTACAACAAGTAACA
CAATCAATATCAATCATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCANCGTTATT
   
Sequence Name 24-K118545w-0022-379-G06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118545w-0022-379-G06C-8409
TACATCTCTTAGGTTACCCTTGGGTGACGTTGAGAAGGTTAAAAAAGATGAAAGACTTTT
GCCGTTTATGTGAAGAATTGGTGATGATGAGAATGGGGTTTATGATTGGAAATCTCTCAC
AGAAGTTTCCTCAATTTTCTATTGATCTCTACTTAGGGCACGAGTTTGAGATT
Primers used for PCR 46wp - 8474, show primer details
   
Sequence Name 45-K118546w-0022-379-G06C-46wp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 46wp: 5'-ATCTGAGTCGGATTCATCATCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K118546w-0022-379-G06C-46wp
CAGCAGCTTTTCACATGTCAAAGTTAGATTTCTAAGAGATACACATGTAAATATGACTAC
CACAAAGCAGTAGCTTAGTAAAAAAAGTAAACATACCAATTTGTTGTTCCATATATTCAT
CTAACTCAGAATCTACACAAACCAAAAAGCAAAAGATTAGCTTCAAGCTTCATAATAGAG
TAAACCAAATCATCAACTAAAGAATTATAAACTCACCAGAATCAATCTCCTCATCCGTGA
GCATCAGAAAAAAGCAGAGCAAAACAAAATCAAGGATAAACAAACAAAGCTTCAAAATTG
CAAGTGAGTATGAATTTAACTAGAAAAAGACAATGAAAGAAAGGATATAGTTCGTCAAAA
GCATCAAAGGTCCTGTAACCAATAAGATAGACACTGCTTGTACTGTTGTGAGAAATCTCA
AACTCGTGCCCTAAGTAGAGATCAATAGAAAATTGAGGAAACTTCTGTGAGAGATTTCCA
ATCATAAACCCATTCTCATCATCACCAATCTTCACATAAACGGCAAAAGTCTCATCTTTT
TTAACCTTCTCAACGTCACCAAGGGTAACCTAAGAGATGTTACAACAAGTAACACAATCA
ATATCAATCATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCACAAATGTAGATATCCGC
   
Sequence Name 25-K118545w-0022-379-G06C-oriL
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K118545w-0022-379-G06C-oriL
TACATCTCTTAGGTTACCCTTGGTGACGTTGAGAAGGTTAAAAAAGATGAGACTTTTGCC
GTTTATGTGAAGATTGGTGATGATGAGAATGGGTTTATGATTGGAAATCTCTCACAGAAG
TTTCCTCAATTTTCTATTGATCTCTACTTAGGGCACGAGTTTGAGATTTCTCACAACAGT
ACAAGCAGTGTCTATCTTATTGGTTACAGGACCTTTGATGCTTTTGACGAACTATATCCT
TTCTTTCATTGTCTTTTTCTAGTTAAATTCATACTCACTTGCAATTTTGAAGCTTTGTTT
GTTTATCCTTGATTTTGTTTTGCTCTGCTTTTTTCTGATGCTCACGGATGAGGAGATTGA
TTCTGGTGAGTTTATAATTCTTTAGTTGATGATTTGGTTTACTCTATTATGAAGCTTGAA
GCTAATCTTTTGCTTTTTGGTTTGTGTAGATTCTGAGTTAGATGAATATATGGAACAACA
AATTGGTATGTTTACTTTTTTTACTAAGCTACTGCTTTGTGGTAGTCATATTTACATGTG
TATCTCTTATGAAATCTAACTTTGACATTGTTGAAAAGCTGCTTTGCCTCAAAATGAGAT
CAATCCTGAAGAAGATGATGAACCGAACTCAG

Last Updated on 06.12.2018 16:11