Sequences from confirmation amplicon


Confirmation sequences for line 878B05 and hit At2g28660 are shown.
Line ID 878B05
Locus At2g28660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12295736
Primers used for PCR 23sv - 8474, show primer details
   
Sequence Name 40-K116654-0022-878-B05C-23sv
GenBank Accession LN503839 [GenBank]
Sequencing primer gene specific primer, 23sv: 5'-ATAATCAAACCTGGTCATCAGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K116654-0022-878-B05C-23sv
AGAGAGAAGATGATGAAGAGACGAAGAAGATGATGAGGCTGAAGAAGTTACGTTGCGGTC
ACGGTAAAGAATCAAATCTTTAGAACGATCCGATGATAACCAGAGGTCCTTATCATCACC
TTTCAAAGACTTATGATCTTTTAAAAGATACCTGGACGATGAGCTTAGCAGAGAACTCCT
TGATATATGTGATTATTAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 15-K116653-0022-878-B05C-8409
GenBank Accession LN493142 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K116653-0022-878-B05C-8409
ATTTCATTGAAATTATAATCACATATATCAAGGAGTTCTCTGCTAAGCTCATCGTCCAGG
TATCTTTTAAAAGATCATAAGTCTTTGAAAGGTGATGATAAGGACCTCTGGTTATCATCG
GATCGTTCTAAAGATTTGATTCTTTACCGTGACCGCAACGTAACTTCTTCAGCCTCATCA
TCTTCTTCGTCTTCTTCATCATCTTTCTCTTCTTCTGTGACCAATGTTTCATCGCCGGCT
CCATCCACTGATGACCAGGTTTGATTAT

Last Updated on 06.12.2018 16:11