Sequences from confirmation amplicon


Confirmation sequences for line 587H06 and hit At2g29400 are shown.
Line ID 587H06
Locus At2g29400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12613733
Primers used for PCR KS33 - 8474, show primer details
   
Sequence Name 88-K033099-022-587-H06C-8409
GenBank Accession LN514818 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K033099-022-587-H06C-8409
CATTTACATTGAATATCCATCAATTGATTTACCTTTTGTCGTAATTGTTAATTTGAAGCA
ATGGTGGTCACTGGATAATCGGTGTCACAGAAAAGGCGACTTCTTTTCTGACGGCTTTAG
AATCTGGAATGAGCACATTAAGCTCTCATCAATGCTCATCATTGCGCCAGCGTTGTCAAA
TTCCCCGCAATAGTTGGGAGCTGAAAATACTGTAACAAGTTGTCTTTCTGCAAAGAACTC
ATACCCATCTTCAACAACCTATAAACAGAGCCAATACACTCAAATTAAGGTTGCACAGTT
TTCATAATAAACTAAATGGCAGATATAACTCAAGTTAAAACCTGGTGGGCACGACAGATA
AGGTCCATGTCATGTTTCTCCAAGAACTCTGCGACTTTGTCAGCTCCAAAAGTGTATGAA
ACACCACGATCATTCATGCCCCAGCCTACGTCTCCACTAGGATCCGACCATAGTAAATCA
CATACCAAACCAGACTCCGGAATATCCATCGGCCGTGCAATATTTCTAATCTGGT
   
Sequence Name 40-K033099-022-587-H06C-KS33
GenBank Accession LN503616 [GenBank]
Sequencing primer gene specific primer, KS33: 5'-GCTGAAAAGTTTGGACCAGATTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K033099-022-587-H06C-KS33
CTGGTTTGGTATGTGATTTACTATGGTCGGATCCTAGTGGAGACGTAGGCTGGGGCATGA
ATGATCGTGGTGTTTCATACACTTTTGGAGCTGACAAAGTCGCAGAGTTCTTGGAGAAAC
ATGACATGGACCTTATCTGTCGTGCCCACCAGGTTTTAACTTGAGTTATATCTGCCATTT
AGTTTATTATGAAAACTGTGCAACCTTAATTTGAGTGTATTGGCTCTGTTTATAGGTTGT
TGAAGATGGGTATGAGTTCTTTGCAGAAAGACAACTTGTTACAGTATTTTCAGCTCCCAA
CTATTGCGGGGAATTTGACAACGCTGGCGCAATGATGAGCATTGATGAGAGCTTAATGTG
CTCATTCCAGATTCTAAAGCCGTCAGAAAAGAAGTCGCCTTTTCTGTGACACCGATTATC
CAGTGACCACCATTGCTTCAAATTAACAATTACGACAAAAGGTAAATCAATTGATGGATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC

Last Updated on 06.12.2018 16:11