Sequences from confirmation amplicon


Confirmation sequences for line 644H11 and hit At2g30240 are shown.
Line ID 644H11
Locus At2g30240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12902751
Primers used for PCR 29fp - 8474, show primer details
   
Sequence Name 26-K111563-0022-644-H11C-29fp
GenBank Accession LN497989 [GenBank]
Sequencing primer gene specific primer, 29fp: 5'-GAGGAAAGATGATCAACCAAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K111563-0022-644-H11C-29fp
ATAATAGACACAATATAGGCTCATCTTTCTTCTACCTTCTCTCACATACCTCTATCTCTC
TCTCTTTGATCTCACCAAAGTCGAAGAACCAATCTATCTTTTGTGTATTTACTGATAAGA
AGAAGTTAGCCTTCATAGCCACAAGTAAAAACAGAGTCCTCTGTTTTCTTCTCTCATTCT
CCCTTACATTTTCTCATCAAAAATGAAAACATAACATTGATAGTTTCCCAAACCTAGTCT
CTCTCCCTCTTTCTACTCGCTTTCTTCGTTTTACTGATAAGGAAAAGACTATGGAGCTTT
CGATGTTTGGCAGAGAAGAATATGATGATGGCTTCAATATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 10-K111556w-0022-644-H11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K111556w-0022-644-H11C-8409
CATTTCATTGAATTATATTGAAGCCTCATCATATTCTTCTCTGCCAACATCGAAAGCTCC
ATAGTCTTTTCCTTATCAGTAAAACGAAGAAAGCGAGTAGAAAGAGGGAGAGAGACTAGG
TTTGGGAAACTATCAATGTTATGTTTTCATTTTTGATGAGAAAATGTAAGGGAGAATGAG
AGAAGAAAACAGAGGACTCTGTTTTTACTTGTGGCTATGAAGGCTAACTTCTTCTTATCA
GTAAATACACAAAAGATAGATTGGTTCTTCGACTTTGGTGAGATCAAAGAGAGAGAGATA
GAGGTATGTGAGAGAAGGTAGAAGAAAGATGAGCCTATATTTGTTGTTTCTATTTATTGT
TTCTTTCTATTATTATGTTTGGTTGATCATCTTTCCT

Last Updated on 06.12.2018 16:11