Sequences from confirmation amplicon


Confirmation sequences for line 625F12 and hit At2g30530 are shown.
Line ID 625F12
Locus At2g30530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13009339
Primers used for PCR 75qa - 8474, show primer details
   
Sequence Name 49-K115481-0022-625-F12C-75qa
GenBank Accession LN506940 [GenBank]
Sequencing primer gene specific primer, 75qa: 5'-TCTGTAATGAAGGATTCTCGCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K115481-0022-625-F12C-75qa
CTCCATAAGAACGCAACCCCACAAAACAATTGGAAGAGCAAATGCAACTATAAAAACCTA
GTCTTTAACCTTAGTTCCTGAAGTTGTCGCAGAAGGTATGCTCTGCTCCATTCTCTTAGG
ACTTTGAGGCAACTGATTAGGATCATCCTCATCCAAAAAAGCCTTAGCTTTACTAGCTAA
ACTTCCCCAAAAGCCTCGTTTTGGTTCATTCAAACTCTTCATAGAAGTATACTCATAAGC
CTTATTCACCTCCTATTCAAACGATTCAATTGAGGCAAAAACAGAGTAAACCCATGAATC
ATTTGGAACATTTCAATTGAAGCAAAAACAGAGTAAACCCATGAATCATTATGAACAATT
AAGCGACACCAGAAAGAGAGAAGAACAAAAGATGAAACCTTGGCAGAAGATGAACCCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATTC
   
Sequence Name 21-K115480-0022-625-F12C-8409
GenBank Accession LN495792 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K115480-0022-625-F12C-8409
CTTTTGTTCTTCTCTCTTTCTGGTGTCGCTTAATTGTTCATAATGATTCATGGGTTTACT
CTGTTTTTGCTTCAATTGAAATGTTCCAAATGATTCATGGGTTTACTCTGTTTTTGCCTC
AATTGAATCGTTTGAATAGGAGGTGAATAAGGCTTATGAGTATACTTCTATGAAGAGTTT
GAATGAACCAAAACGAGGCTTTTGGGGAAGTTTAGCTAGTAAAGCTAAGGCTTTTTTGGA
TGAGGATGATCCTAATCAGTTGCCTCAAAGTCCTAAGAGAATGGAGCAGAGCATACCTTC
TGCGACAACTTCAGGAACTAAGGTTAAAGACTAGGTTTTTATAGTTGCATTTGCTCTTCC
AATTGTTTTGTGGGGTTGCGTTCTTATGGAGTAGAAATCCATTTCCATGTTTCAGGAAGC
TGGTCAGACTGGTAGGAAAAGCGAGA

Last Updated on 06.12.2018 16:11