Sequences from confirmation amplicon


Confirmation sequences for line 529C05 and hit At2g30615 are shown.
Line ID 529C05
Locus At2g30615 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13041914
Primers used for PCR 17vz - 8474, show primer details
   
Sequence Name 46-K118356-0022-529-C05C-17vz
GenBank Accession LN506005 [GenBank]
Sequencing primer gene specific primer, 17vz: 5'-GAGACATGCGTTTAACATGTGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K118356-0022-529-C05C-17vz
CTCTGGATCTTCACACTGAACAGTTTCGTGATGTCCCAACACCGCCCACACCCCGGGAAC
CGAGCGAGCTAGTTAACCTTGAAGATCGTCTAGCCTTGGTTAAAACATATTATGGAGTTT
CTCATTGGGAAGTAAGGATATGGAGCTTGGATCCACAGGAAGATACATGGACCAAGACGT
ACTCATTTTGTTTATTCCCTAGAGCTCTTCACCCTTGGATATGGAGGTTGTGGTGTAGAC
CAGTGACAGTTTCTAAGCAAGGGAATCTTTTCTTCCATGACAGTGAAAAGAGGCTGTTCA
AATATAATCCAGAAACAAACGAAGTCCATTTTATCGCTGCAGACATTCGTGTTTTATCTC
ATTTCGTAGAGAATATGGTTCCACTTCGACATTCGGATTCTGCTCCAAAGTAAATCATTT
GCCGCCGGACTCATGGATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATATCCGCA
   
Sequence Name 46-K118355-0022-529-C05C-8409
GenBank Accession LN506004 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K118355-0022-529-C05C-8409
CCGGCGGCAAAGATTTACTTTGGAGCAGAATCCGAATGTCGAAGTGGAACCATATTCTCT
ACGAAATGAGATAAAACACGAATGTCTGCAGCGATAAAATGGACTTCGTTTGTTTCTGGA
TTATATTTGAACAGCCTCTTTTCACTGTCATGGAAGAAAAGATTCCCTTGCTTAGAAACT
GTCACTGGTCTACACCACAACCTCCATATCCAAGGGTGAAGAGCTCTAGGGAATAAACAA
AATGAGTACGTCTTGGTCCATGTATCTTCCTGTGGATCCAAGCTCCATATCCTTACTTCC
CAATGAGAAACTCCATAATATGTTTTAACCAAGGCTAGACGATCTTCAAGGTTAACTAGC
TCGCTCGGTTCCCGGGGTGTGGGCGGTGTTGGGACATCACGAAACTGTTCAGTGTGAAGA
TCCAGAGCTAGAATCTTGAAACGCATGTTAAGATGTGTCACTCACATGTT

Last Updated on 06.12.2018 16:11