Sequences from confirmation amplicon


Confirmation sequences for line 871D07 and hit At2g30890 are shown.
Line ID 871D07
Locus At2g30890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13148977
Primers used for PCR 42dl - 8474, show primer details
   
Sequence Name 30-K110172-0022-871-D07C-42dl
GenBank Accession LN499765 [GenBank]
Sequencing primer gene specific primer, 42dl: 5'-GCAAAGATGCTTCTCTCTTCATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K110172-0022-871-D07C-42dl
TTACACTTTTTCGTGATTTTAATTAGTACTCCATTAGTAATTAGTAGGGCCTTTTAGGTT
ATTCTAGATTGTTAATCACATTGATAATCATGGAAACTTGAAGGCCAAGCTAAAGCATCT
ATCTTCTTCCTCTTTATTTTTAGCTTTAACGACTTTCTGACTTTCTCTTTCTCTCATTCT
GATACTTAACTAAGAAACCTCACCTACTAGCTAGTCAATTTTGTTACAAAACAATATTTG
ATAATTAAACAGCAGCAAGTACTTTTAACTATTGTTATCTATCAACAAGCGGCTCATAGT
TTACTTGGAATATTAAGTGAGCAATTAACAATGTGATTAATTTATGAAGCAGATGGTTGC
AGTGATACTTGTGATTAACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCACGATTA
   
Sequence Name 14-K110171-0022-871-D07C-8409
GenBank Accession LN492555 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K110171-0022-871-D07C-8409
TTCGGAATGAGCCATTTACATTGTTAATCACAAGTATCACTGCAACCATCTGCTTCATAA
ATTAATCACATTGTTAATTGCTCACTTAATATTCCAAGTAAACTATGAGCCGCTTGTTGA
TAGATAACAATAGTTAAAAGTACTTGCTGCTGTTTAATTATCAAATATTGTTTTGTAACA
AAATTGACTAGCTAGTAGGTGAGGTTTCTTAGTTAAGTATCAGAATGAGAGAAAGAGAAA
GTCAGAAAGTCGTTAAAGCTAAAAATAAAGAGGAAGAAGATAGATGCTTTAGCTTGGCCT
TCAAGTTTCCATGATTATCAATGTGATTAACAATCTAGAATAACCTAAAAGGCCCTACTA
ATTACTAATGGAGTACTAATTAAAAATCACGAAAAAGTTGTTAATTGGATGCAATCTTTA
TTGTTTAGATGAAGAGAGAAGCATCTTTG

Last Updated on 06.12.2018 16:11