Sequences from confirmation amplicon


Confirmation sequences for line 783A05 and hit At2g31470 are shown.
Line ID 783A05
Locus At2g31470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13408801
Primers used for PCR BH55 - 8474, show primer details
   
Sequence Name 34-K104383-0022-783-A05C-BH55
GenBank Accession LN501404 [GenBank]
Sequencing primer gene specific primer, BH55: 5'-ATATGAAAACTGGCAGCTAAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K104383-0022-783-A05C-BH55
TGTCGAGAAGAAAACAACTCTCTGTGTTTTTGGCAGGCGAACAAGAGCCTCGGGCGAGCA
CAAGATTTGGTCAAGTACAACTCCGTGAAATAGGGTAGGCGGAGTATGGAGGCCCAAAGC
TTTGATACGCAACGACATCTTGCTATAGACTTCACAGGCGACCTTGAGAATATCTCGATA
ACGAGATCAATTGGGATTGGTTCAAAGTTTTCGTCGCGTCCAAGGATGGTTTGTCGAGCC
ACGGACACATTCTGTCGCCGTGATTTCATGTTGCAGTAATCAAATTCTTTTGGAACGATA
TGAAGAATAGGGTTAGAAAAAGTAAATATAAAAAGTGATATAGTTTGCGTGTCGTTATTT
TTCCATTAAACACGGCGCAGGATTTATCATGACTAATAAAAAAAGGTTAACATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 42-K104384-0022-783-A05C-8409
GenBank Accession LN504452 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K104384-0022-783-A05C-8409
GCCATTTACATTGAATATGTTAACCTTTTTTTATTAGTCATGATAAATCCTGCGCCGGTG
TTTAATGGAAAAATAACGACACGCAAACTATATCACTTTTTATATTTACTTTTTCTAACC
CTATTCTTCATATCGTTCCAAAAGAATTTGATTACTGCAACATGAAATCACGGCGACAGA
ATGTGTCCGTGGCTCGACAAACCATCCTTGGACGCGACGAAAACTTTGAACCAATCCCAA
TTGATCTCGTTATCGAGATATTCTCAAGGTCGCCTGTGAAGTCTATAGCAAGATGTCGTT
GCGTATCAAAGCTTTGGGCCTCCATACTCCGCCTACCCTATTTCACGGAGTTGTACTTGA
CCAAATCTTGTGCTCGCCCGAGGCTCTTGTTCGCCTGCCAAAAACACAGAGAGTTGTTCT
TCTTCTCGACACCTCAGCCTCATAATCCTAATGAGAGCTCGTCTCCTTTAGCTGCCATTT
TTCAT

Last Updated on 06.12.2018 16:11