Sequences from confirmation amplicon


Confirmation sequences for line 800B03 and hit At2g33170 are shown.
Line ID 800B03
Locus At2g33170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14057071
Primers used for PCR EU71 - 8474, show primer details
   
Sequence Name 76-K103762-0022-800-B03C-EU71
GenBank Accession LN513386 [GenBank]
Sequencing primer gene specific primer, EU71: 5'-ATGACTCTAACCAGTTTTCTCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K103762-0022-800-B03C-EU71
GCATTTGAGAGCTGTGACCCTAGCCCTCCTCTTGGCCTCACATTTCATCTCTAAAAGCGG
GTTCTGCTCGGAGGGGGAGAATCATCATCATCGTGTCTTCAGTCATTGGCGGTATCTCTC
TTTTACTAATCGCGATTGTCGTTCATTTCCTAAGAAATCCCGTGGAACCAACGGCTCCTT
ATGTGCACGACAAGGAACCCTTTTTTCAAGAATCCGACATTTACTTTGTCCCGAAAGAAC
GATTCACAGTCAAAGACATTTTGGAAGCAACAAAAGGCTTCCACGATAGCTACATTGTCG
GTAGAGGCGCTTGCGGGACCGTTTACAAAGCAGTTATGCCTTCAGGTAAAACAATAGCAG
TTAAAAAACTCGAATCCAACAGAGAAGGCAACAACAACAACAGCAACAACACTGATAACA
GTTTCCGCGCCGAGATTCTAACACTAGGCAAAATCCGTCACAGGAACATTGTCAGGCTAT
ACAGTTTCTGTTACCACCAAGGATCAAACTCGAACCTCTTGCTCTACGAATACATGTCTC
GGGGAAGCCTTGGCGAGTTACTTCACGGCGGGAAATCACATAGTATGATGGTCAATTGCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCA
   
Sequence Name 67-K103763-0022-800-B03C-8409
GenBank Accession LN511799 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K103763-0022-800-B03C-8409
CATTTACATTGAATATATCCTGCAATTGACCATCATACTATGTGATTTCCCGCCGTGAAG
TAACTCGCCAAGGCTTCCCCGAGACATGTATTCGTAGAGCAAGAGGTTCGAGTTTGATCC
TTGGTGGTAACAGAAACTGTATAGCCTGACAATGTTCCTGTGACGGATTTTGCCTAGTGT
TAGAATCTCGGCGCGGAAACTGTTATCAGTGTTGTTGCTGTTGTTGTTGTTGCCTTCTCT
GTTGGATTCGAGTTTTTTAACTGCTATTGTTTTACCTGAAGGCATAACTGCTTTGTAAAC
GGTCCCGCAAGCGCCTCTACCGACAATGTAGCTATCGTGGAAGCCTTTTGTTGCTTCCAA
AATGTCTTTGACTGTGAATCGTTCTTTCGGGACAAAGTAAATGTCGGATTCTTGAAAAAA
GGGTTCCTTGTCGTGCACATAAGGAGCCGTTGGTTCCACGGGATTTCTTAGGAAATGAAC
GACAATCGCGATTAGTAAAAGAGAGATACCGCCAATGACTGAAGACACGATGATGATGAT
TCTCCCCCTCCGAGCAGAACCCGCTTTTAGAGATGAAATGTGAGGCCAAGAGGAGTGGCT
AGGGTCACAGCTTCTCAAATGGCCGCCACAGAGTCCTTTGTTTCCGAGAAAACTGG

Last Updated on 06.12.2018 16:11