Sequences from confirmation amplicon


Confirmation sequences for line 200G06 and hit At2g33490 are shown.
Line ID 200G06
Locus At2g33490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14186862
Primers used for PCR 41fm - 8474, show primer details
   
Sequence Name 29-K111117-0022-200-G06C-41fm
GenBank Accession LN499343 [GenBank]
Sequencing primer gene specific primer, 41fm: 5'-AAATTGGAGGACCATATACTGCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K111117-0022-200-G06C-41fm
ATCTTCTCTGCTTTTCCTTTCAATGACGCAGACAAATTCTCAGGAAGCTCATAGTTCAAG
GAATTTAATTTCTTTCATCTTTACCACGAAAACCACCCAATTTATTTCTCTCTTACCAGT
CTTTGTCTTTTTATTTATTTACCAGGAAAATGAGGAAGGAAATTACAGAAAATCTCATTC
GTTTAGGAGAGATGTAAGGGCAGTGAGCCAATCAGCACCACTTTTTCCCGAGAACCGCAC
AACTCCTCCTTCAGAAAAGCTGTTACGGATGCGATCAACTCTGACACGGAAATTCAACAC
TTATGCATTGCCAACTCCTGTGGAAACAACGAGAAGTCCCTCATCTACTACAAGTCCAGG
TCACAAAAACGTGGGGTCATCTAATCCTACAAAGGCAATTACAAAGCAGATTTGGTATTC
ATCCCCACTTGAAACTCGCGGACCTGCAAAGGTTTCCTCAAGATCAATGGTAGCCTTGAA
AGAACAAGTCCTGAGAGAGAGTAACAAGAATACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 13-K111116-0022-200-G06C-8409
GenBank Accession LN492095 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K111116-0022-200-G06C-8409
TTCTTTCAGGCTACCATTGATCTTGAGGAAACCTTTGCAGGTCCGCGAGTTTCAAGTGGG
GATGAATACCAAATCTGCTTTGTAATTGCCTTTGTAGGATTAGATGACCCCACGTTTTTG
TGACCTGGACTTGTAGTAGATGAGGGACTTCTCGTTGTTTCCACAGGAGTTGGCAATGCA
TAAGTGTTGAATTTCCGTGTCAGAGTTGATCGCATCCGTAACAGCTTTTCTGAAGGAGGA
GTTGTGCGGTTCTCGGGAAAAAGTGGTGCTGATTGGCTCACTGCCCTTACATCTCTCCTA
AACGAATGAGATTTTCTGTAATTTCCTTCCTCATTTTCCTGGTAAATAAATAAAAAGACA
AAGACTGGTAAGAGAGAAATAAATTGGGTGGTTTTCGTGGTAAAGATGAAAGAAATTAAA
TTCCTTGAACTATGAGCTTCCTGAGAATTTGTCTGCGTCATTTGAAAGGAAAAGCAGAGA
AGATGTGATCAAAAATAAAGCATGGTTGTGTTACCTGTGCAGTATAT

Last Updated on 06.12.2018 16:11