Sequences from confirmation amplicon


Confirmation sequences for line 122B02 and hit At2g34660 are shown.
Line ID 122B02
Locus At2g34660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14609499
Primers used for PCR 04fp - 8474, show primer details
   
Sequence Name 49-K111394-0022-122-B02C-04fp
GenBank Accession LN506881 [GenBank]
Sequencing primer gene specific primer, 04fp: 5'-TCAAATCTTCACTCATCCATCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K111394-0022-122-B02C-04fp
ATATGAGATTGTAGAAAAGGGGTCCATGACTCTTTGGAGTTCCTGCATCAGTCCACTCAC
TCAACCACGTGCTGCTAGTAACCCGAAATACTTCTGTTAAGACGTAACATAAAAGGAGCA
TCATCACTACCCATGCCCCTCCAAGTGCATCCTGGTACCTGAAAACATTGAAAGCCTGGA
AAAGTCATTCTGATATCCCGCAAAGGTATAGTTCAGCCTAAAAACATAAGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 33-K111393-0022-122-B02C-8409
GenBank Accession LN501045 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K111393-0022-122-B02C-8409
CTTTGCGGGATATCAGAATGACTTTTCCAGGCTTTCAATGTTTTCAGGTACCAGGATGCA
CTTGGAGGGGCATGGGTAGTGATGATGCTCCTTTTATGTTACGTCTTAACAGAAGTATTT
CGGGTTACTAGCAGCACGTGGTTGAGTGAGTGGACTGATGCAGGAACTCCAAAGAGTCAT
GGACCCCTTTTCTACAATCTCATATATGCACTTCTCTCGTTTGGACAGGTATGAGTTATG
TTTGCTTGATGGATGAGTGAAGATTTGA

Last Updated on 06.12.2018 16:11