Sequences from confirmation amplicon


Confirmation sequences for line 013A03 and hit At2g35150 are shown.
Line ID 013A03
Locus At2g35150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14818479
Primers used for PCR 01kb - 8474, show primer details
   
Sequence Name 40-K113916-0022-013-A03C-01kb
GenBank Accession LN503778 [GenBank]
Sequencing primer gene specific primer, 01kb: 5'-CTCAATGTGTGACAACTTTCCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K113916-0022-013-A03C-01kb
GTTAACAAGAGAAACTTTCTCTAACTTTCGTAATTGGGTCTTATAAAATTTTATGCAATT
ATGATTTTACCCTTTTACTACTTTTCATTAGCTTTCACGAATCTATTTTGACAAGAGAAA
TCATTAGAGGTAAACATGCTTTTTGGTCAAGGGCCTTAACAGTTCCACCAATCAAGCTCA
AAAGTTGTACTTAACCGACATCTTCTGTGAAAACATATAATTACATGTACAAATCAAAAC
TCAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGA
   
Sequence Name 12-K113915-0022-013-A03C-8409
GenBank Accession LN491662 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K113915-0022-013-A03C-8409
GAAACCGTTAAGGCCCTCGACCAAAAAGCATCGTTTACCTCTAATGATTTCTCTTGTCAA
AATAGATTCGTGAAAGCTAATGAAAAGTAGTAAAAGGGTAAAATCATAATTGCATAAAAT
TTTATAAGACCCAATTACGAAAGTTAGAGAAGTTTCTCTTGTTAACCAGAAACACTGAAT
TAAGCATTGACATAAAGATATTTTTACTCTTGTATGTAATATTTACCATCTACTATTTCA
ATAATTGCACTCATCGCTAGTCACTTTATCTTAGTGCCAGTGGAAAG

Last Updated on 06.12.2018 16:11