Sequences from confirmation amplicon


Confirmation sequences for line 154H01 and hit At2g37080 are shown.
Line ID 154H01
Locus At2g37080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15581564
Primers used for PCR 64zd - 8474, show primer details
   
Sequence Name 32-K124484-0022-154-H01C-64zd
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 64zd: 5'-TATCATCTACCATGAACGAGGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K124484-0022-154-H01C-64zd
GGACGAAGGCTTACTGTGCGCCACCGCCCACGCCAGCGGATAGCCGATCAGTAAACAGCA
AAATGTAGATGTCCGCAGCGTTATTATATCAGGCGCATGAAATTGTGTCGGGAACTGAAA
AGCAATTAGAGATAGCCAACTTGACGCTTGAGATGTTACGTTCAGATGGTATGAAGATGT
CTGAAGCTTGTAATTCACTAACAACTGAGCTAGAGCAGTCTAAATCTGAGGTAAGGTCAC
TAGAGCAACTTGTGAGACAGCTTGAGGAAGAAGATGAAGCCCGAGGAAATGCTAACGGGG
ACTCATCATCAGTGGAAGAGCTCAAGGAAGAGATAAATGTTGCAAGGCAAGAGATTAGCC
AGTTGAAATCTGCAGTGGAAGTAACAGAGAGAAGGTACCATGAAGAGTATATCCAGAGCA
CGTTGCAGATCAGAACTGCTTATGAACAAGTCGATGAAGTAAAATCCGGATATGCACAGA
GAGAGGCTGAGTTGGGGGAAGAACTGAAGAAAACCAAAGCTGAAAGAGATTCTTTGCATG
AACGGTTGATGGATAAGGAAGCTAAGCTCAGGATACTCGTCGATGAAAACGAGATACTGA
ACTCGAAAATCAAAGAAAAAGAAGAAGTATACTTAAACTTGGAGAACAGCTTGAACCAGA
ACGAGCCTGAGGATACCGGTGAACTCAAGAAGCTAGAATCCGATGTGATGGAGTTGAGAG
CAAATCTAATGGACAAGGAGATGGAGCTACAGAGCGTAATGTCGCAATACGAGAGTCTCA
AGAGCGAAATGGAGACAATGCAGAGTGAGAAGAACAAGGCTATCGA
   
Sequence Name 08-K124483-0022-154-H01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K124483-0022-154-H01C-8409
GCCATTTACATTGAATAATCATTTCTGACTTTTCTTCAGTAACACACCAATTTTCTTCAG
CATACTTCCATTCTTCTTCTTTGGTGATGACAATTAATCATCAGTTTCACCCATATAAGG
CGACATGTTGACGTTTCTCCTCCTCAACGGACTCTCTAACGATCCTGTTCTCTCCACGTA
CTTCCCGTTACTGTTATTGTTGTTGTTACCACCAGAGAGCATAGTAGCTGCGGCTTCAGC
TGCTTTCCTCCATTGATCACACTGAACCTTGAGTCTCCTCAGCTCAGCTTCTAGTTCGGT
GTTGGTGACTTGTGCCGCCCCTAGCTGTTCTGTCGCATTTTCGGCTCTCTTCCCGCTCTT
ATCTGCTTCCTCTGTCAGACTCCCGAGCTTTGCTAAGGCCTCGTCGATAGCCTTGTTCTT
CTCACTCTGCATTGTCTCCATTTCGCTCCTGAGACTCTCGTATTGCGACATTACGCTCTG
TAGCTCCATCTCCTTGTCCATTAGATTTGCTCTCAACTCCATCACATCGGATTCTAGCTT
CTTGAGTTCACCGGTATCCTCAGGCTCGTTCTGGTTCAAGCTGTTCTCCAAGTTTAAGTA
TACTTCTTCTTTTTCTTTGATTATCGAGTTCAGAATCTCGTTTTCATCGACGAATATCCT
GA

Last Updated on 06.12.2018 16:11