Sequences from confirmation amplicon


Confirmation sequences for line 423C02 and hit At2g38230 are shown.
Line ID 423C02
Locus At2g38230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16012058
Primers used for PCR TF05 - 8474, show primer details
   
Sequence Name 21-K024507-022-423-C02C-8409
GenBank Accession LN495502 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K024507-022-423-C02C-8409
TACGATGACGAGGTTTTCACTTACGCGAAAAAGATCGCTGCGCCGTATGATTTGGTTGTG
CAGACTAAGGAGCTTGGGAGGTTACCGGTGGTTCAGTTCGCTGCTGGAGGAGTGGCGACG
CCGGCGGATGCGGCGTTGATGATGCAGTTGGGATGTGATGGAGTGTTTGTTGGGTCGGGT
GTTTTCAAGAGTGGAGATCCGGTGAAGAGGGCTAAGGCTATTGTTCAGGCGGTTACGAAT
TATAGAGACGCGGCGGTGTTGGCGGAGGTGAGCTGTGGTTTAGGTGAAGCCATGGTTGGT
CTTAATTTGGATGATAAGGTTGAGAGGTTCGCTAGTCGTTCTGAGTAACCAATCAAATTT
CAGATGTTTTTATCCATAACGTTTGTCACTTTAATATGTATCCACAAACCAATATTCTCG
ATTTATTCAAGATTTTATAGAAAAAAATTGGAAATGAGGAGTTT
   
Sequence Name 22-K024507-022-423-C02C-TF05
GenBank Accession LN495949 [GenBank]
Sequencing primer gene specific primer, TF05: 5'-CTAGCCAACAAAACTCCTCATTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K024507-022-423-C02C-TF05
ATTTTTTTCTATAAAATCTTGAATAAATCGAGAATATTGGTTTGTGGATACATATTAAAG
TGACAAACGTTATGGATAAAAACATCTGAAATTTGATTGGTTACTCAGAACGACTAGCGA
ACCTCTCAACCTTATCATCCAAATTAAGACCAACCATGGCTTCACCTAAACCACAGCTCA
CCTCCGCCAACACCGCCGCGTCTCTATAATTCGTAACCGCCTGAACAATAGCCTTAGCCC
TCTTCACCGGATCTCCACTCTTGAAAACACCCGACCCAACAAACACTCCATCACATCCCA
ACTGCATCATCAACGCCGCATCCGCCGGCGTCGCCACTCCTCCAGCAGCGAACTGAACCA
CCGGTAACCTCCCAAGCTCCTTAGTCTGCACAACCAAATCATACGGCGCAGCGATCTTTT
TCGCGTAAGTGAAAACCTCGTCATCGTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
T

Last Updated on 06.12.2018 16:11