Sequences from confirmation amplicon


Confirmation sequences for line 515H05 and hit At2g38440 are shown.
Line ID 515H05
Locus At2g38440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16097579
Primers used for PCR 21hz - 8474, show primer details
   
Sequence Name 44-K112562-0022-515-H05C-21hz
GenBank Accession LN505234 [GenBank]
Sequencing primer gene specific primer, 21hz: 5'-AGTATGGTGGGTGCTATGGATAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K112562-0022-515-H05C-21hz
GCAGGAAATGTGGCAAATGTTAACATGAATGGGGGTTTTATTGAAAAAGATATAGAGACA
GTGCCTGAATCTACATACAATGAGGTCCGTGGAACAACTATTACACAGGATTCACAAACG
GTTTTGAACGGGAAGCCAGGATTTTTTCAACAGAGGAGTTATTCTGAAGATTTAACCAGT
GAGGCTGACAATTATGTGGACGCACCAGCCACCATGGAGTCGGAAACAGAAACAGACGAT
GAGTGTAGACCTAAGAGTAGATCAGATACTTTGAAGGATGGGAACCATCATATATATTCT
GATGCGGTTGAAGAGAGAATGGAGGACCCACCTCAGTTTTCATTTTCTCATTCAAATGGA
AACACACCAGTTTCGGAAAATGGGCGAAGCTCGTTTGGGAAAAAGAGCACTAGTTATTCT
TACCAATGTAGACTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 20-K112561-0022-515-H05C-8409
GenBank Accession LN495276 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K112561-0022-515-H05C-8409
TCATTGAATATATCCTGAAGTCTACATTGGTAAGAATAACTAGTGCTCTTTTTCCCAAAC
GAGCTTCGCCCATTTTCCGAAACTGGTGTGTTTCCATTTGAATGAGAAAATGAAAACTGA
GGTGGGTCCTCCATTCTCTCTTCAACCGCATCAGAATATATATGATGGTTCCCATCCTTC
AAAGTATCTGATCTACTCTTAGGTCTACACTCATCGTCTGTTTCTGTTTCCGACTCCATG
GTGGCTGGTGCGTCCACATAATTGTCAGCCTCACTGGTTAAATCTTCAGAATAACTCCTC
TGTTGAAAAAATCCTGGCTTCCCGTTCAAAACCGTTTGTGAATCCTGTGTAATAGTTGTT
CCACGGACCTCATTGTATGTAGATTCAGGCACTGTCTCTATATCTTTTTCAATAAAACCC
CCATTCATGTTAACATTTGCCACATTTTCCTGCTCACTTGGGAATGAAACCTCTGCTCTG
CTTCCTCCATGAGACTTATCCATAGCACCNACCATAC

Last Updated on 06.12.2018 16:11